一个具有S位点的水稻类受体激酶OsSRL参与干旱胁迫反应  

OsSRL,a Receptor-like Kinase with S-locus Site,Is Involved in Drought Tolerance in Rice

作　　者：许凤莲[1] 李姣姣[1] 杜春晖[1] 王娜[1] 张素巧[1] 

机构地区：[1]河北师范大学生命科学学院,河北省分子细胞生物学重点实验室,石家庄050016

出　　处：《植物学报》2012年第5期474-482,共9页Chinese Bulletin of Botany

基　　金：国家自然科学基金(No.30770216;No.31170292);河北省自然科学基金(No.C2010000390)

摘　　要：植物在进化过程中针对干旱、高盐和高低温等逆境胁迫形成了多种适应机制,植物类受体激酶作为重要的细胞信号传递分子在植物生长和抗逆境胁迫中发挥着重要功能。该文发现一个具有S位点的类受体激酶基因OsSRL可能参与水稻(Oryzasativa)的干旱胁迫反应。利用RNAi技术降低OsSRL的表达水平后,转基因植株抗旱性增强,并表现出幼苗存活率、叶绿素含量及鲜重增加等表型。进一步的研究表明30%PEG和100μmol·L-1ABA可诱导OsSRL基因表达,利用RNAi降低其表达导致干旱诱导基因RAB16A及LEA3表达水平明显增加。表达模式分析发现OsSRL在胚芽、胚根、根、茎节以及花中表达。以上结果表明,OsSRL表达水平的降低增强植物的干旱耐受性,其作为一个S-位点样类受体激酶可能参与了水稻对干旱胁迫的反应。Plants have developed various adaptive mechanisms to drought, salt, temperature and other stresses. Receptor-like kinases play significant roles in plant growth regulation and responses to stresses. We found that a receptor-like kinase with an S-locus domain, OsSRL, might be involved in drought stress response in rice. Downregulation of OsSRL expression by RNA interference produced enhanced drought tolerance and increased seedling survival rate, chlorophyll content and fresh weight as compared with control plants after drought stress treatment. The expression of OsSRL was induced by 30% PEG and 100 iJmol.L-1 abscisic acid. Knocking down OsSRL expression enhanced the ex- pression of drought-response genes RAB16A and LEA3. OsSRL was expressed in plumule, radicula, root, node, and flower. These data suggest that OsSRL, as a receptor kinase with an S-locus domain, may play roles in drought stress tolerance in rice.

关 键 词：干旱胁迫 类受体激酶 水稻 RNA干涉 S-位点 

分 类 号：S511[农业科学—作物学] Q945.78[生物学—植物学]