家蚕ABP与ABPX基因定位与表达分析  

Mapping and expression analysis of ABP and ABPX genes of the silkworm,Bombyx mori

作　　者：张瑶[1,2] 张升祥[1] 路国兵[1,3] 徐世清[4] 崔为正[1] 

机构地区：[1]山东农业大学林学院,山东泰安271018 [2]山东农业大学生命科学学院,山东泰安271018 [3]山东省淄博市林业局,山东淄博255100 [4]苏州大学基础医学与生命科学学院,江苏苏州215123

出　　处：《昆虫学报》2012年第8期895-902,共8页Acta Entomologica Sinica

基　　金：现代农业产业技术体系建设专项资金(CARS-22);山东省自然科学基金项目(ZR2011CM013)

摘　　要：气味结合蛋白(odorant binding proteins,OBPs)在昆虫与外界环境化学信息交流过程中起着重要作用,对昆虫的觅食、求偶、繁殖具有重要意义。触角结合蛋白(antennal-binding protein,ABP)是OBP家族中的重要成员之一。为进一步探明家蚕Bombyx mori ABP与ABPX基因的结构、表达及功能,本研究利用染色体定位、基因分析及半定量表达分析方法对其进行了研究。染色体定位分析表明,BmABP和BmABPX分别位于家蚕第5和第26染色体上,基因结构差异较大,可能功能上有较大差异。对家蚕胚胎、幼虫和成虫不同发育阶段的雌、雄虫多种组织进行基因表达谱分析发现,BmABP在家蚕发育的各个虫态、多种组织器官中都有较高表达,无时间特异性和组织特异性;BmABPX在不同发育时期和不同组织间差异显著(P<0.05),相对表达量以触角中最高,其他非嗅觉组织中也多有表达,性别间差异不大。结果提示,BmABP和BmABPX除了具有嗅觉相关功能外,很可能还具有其他未知的生理功能。Odorant binding proteins （OBPs） in insects play an important role in foraging, courtshipping, multiplying and chemical communication with the environments. Antennal-binding protein （ABP） is one of important groups of OBP family. We analyzed the first reported ABP and ABPX genes in the silkworm Bombyx mori using chromosome mapping and semi-quantitative RT-PCR for further understanding of the expression and function of ABP/ABPX. Chromosome mapping showed that BmABP and BmABPX were respectively organized in chromosome 5 and 26 with different gene structure, suggesting that they probably possess different functions. Analysis of the expression profiles in different tissues of males and females during the embryonic, larval and adult stages revealed that BmABP possessed high expression level in a variety of tissues and organs with no time- and tissue-specificity, while BmABPX possessed conspicuous temporal and spatial expression differences （P 〈 0.05）, with the highest relative expression level in the antenna, and lower relative expression levels in most other non-olfactory tissues without significant sex differences. The results suggest that these two genes are more likely to have other functions undiscovered besides olfactory-related functions.

关 键 词：家蚕 气味结合蛋白 ABP基因 ABPX基因 表达分析 染色体定位 

分 类 号：Q966[生物学—昆虫学] S881.2[农业科学—特种经济动物饲养]