蝶蛹金小蜂热激蛋白家族基因表达与热保护功能  

Prokaryotic expression and thermo-protective function of heat shock proteins from Pteromalus puparum (Hymenoptera:Pteromalidae)

作　　者：王欢[1,2] 李凯[3] 方琦[2] 叶恭银[2] 

机构地区：[1]上海农林职业技术学院,上海201699 [2]浙江大学昆虫科学研究所,杭州310058 [3]东华大学生物科学与技术研究所,上海201620

出　　处：《昆虫学报》2012年第8期903-910,共8页Acta Entomologica Sinica

基　　金：国家自然科学基金杰出青年基金项目(31025021);上海市教育发展基金会晨光计划项目(10CGB18);上海市教委优秀青年教师科研专项基金(nlz10010)

摘　　要：作为分子伴侣,热激蛋白可起修复变性蛋白与阻止其他蛋白质聚集的作用。为进一步理解蝶蛹金小蜂Pteromalus puparum热激蛋白家族的分子伴侣功能,本研究对来自该寄生蜂的热激蛋白Pphsp90,Pphsp70,Pphsc70,Pphsp60,Pphsp40和Pphsp20的基因在大肠杆菌Escherichia coli BL21菌株中进行了过表达。结果表明:除Pphsp40外,其余5个基因均得到高效表达,且表达的重组热激蛋白在高温下(80℃)具可溶性与热稳定性。其中,Pphsp20与Pphsp90在大肠杆菌中的表达显著提高了高温下(50℃,1h)细胞的存活率。离体活性实验证实,利用纯化的融合蛋白Pphsp20可减少高温下荧光素酶的聚集现象。据此认为,Pphsp20与Pphsp90均具有大肠杆菌细胞的热保护功能,但Pphsp20可以单独发挥作用,而Pphsp90可能需其他因子协同作用才有保护活性。Acting as molecular chaperones, heat shock proteins （Hsps） could help fixing denatured proteins and preventing aggregation of other proteins. In order to better understand the function of molecular chaperones of Hsp families in the parasitoid Pteromalus puparum, Pphsp90, Pphsp70, Pphsc70, Pphsp60, Pphsp40 and Pphsp20 genes from this parasitoid were over-expressed in Escherichia coli. The results showed that the five genes except Pphsp40 were highly expressed and these expressed recombinant Pphsps were soluble and stable under high temperature （80~C）. In vivo experiments, Pphsp20 and Pphsp90 significantly increased the survival rate of E. coli cells under high temperature （50~C, 1 h）. In vitro protection study verified that only purified recombinant Pphsp20 could reduce the aggregation of luciferase under high temperature. The results suggest that both Pphsp20 and Pphsp90 may be involved in thermo-protective role in E. coli, requires the cooperation with other cofactors. and Pphsp20 may work independently while Pphspg0

关 键 词：蝶蛹金小蜂 热激蛋白 基因表达 蛋白热稳定性 蛋白纯化 

分 类 号：Q966[生物学—昆虫学]