小菜蛾不同地理种群遗传多样性的ISSR标记研究  

Genetic diversity of different geographical populations of Plutella xylostella (Lepidoptera:Plutellidae) from China based on ISSR analysis

作　　者：朱勋[1,2] 杨家强[2] 吴青君[2] 李建洪[1] 王少丽[2] 郭兆将[2] 刘雅婷[2] 张友军[2] 杨峰山[3] 

机构地区：[1]华中农业大学植物科学技术学院,武汉430070 [2]中国农业科学院蔬菜花卉研究所,北京100081 [3]黑龙江大学生命科学学院,微生物学黑龙江省高校重点实验室,哈尔滨150080

出　　处：《昆虫学报》2012年第8期981-987,共7页Acta Entomologica Sinica

基　　金："十二五"科技支撑计划课题(2012BAD19B06);农业部公益性行业专项(200803001);蔬菜有害生物控制和优质栽培北京市重点实验室资助课题;哈尔滨市科技创新人才研究专项资金优秀学科带头人项目(2007RFXXN048);黑龙江教育厅面上项目(11511273)

摘　　要：为阐明小菜蛾Plutella xylostella不同地理种群的遗传多样性,应用ISSR技术对我国小菜蛾8个地理种群的遗传多样性进行了研究分析。15条引物扩增出395条ISSR条带,其中多态性条带占89.11%,全部个体显示了各自独特的ISSR图谱。ISSR标记的遗传多样性分析结果表明:小菜蛾无论在物种水平上(P=89.11%,H=0.2706,I=0.4286),还是在种群水平上(P=88.80%,H=0.2759,I=0.4349)都表现出较高的遗传多样性。其中,北京南口种群内遗传变异最大,海南海口和甘肃兰州种群内遗传变异最小,南方地区(云南、湖北)小菜蛾种群遗传多样性明显高于北方地区(北京、天津、山东、黑龙江、甘肃)种群。据种群变异来源分析,有5.66%的遗传变异来自种群间,94.34%的变异来源于种群内(N_m=8.3399),不同地理种群间没有明显的遗传分化。本文有关小菜蛾不同地理种群基因流动和遗传变异的研究为小菜蛾抗药性的控制及田间种群的综合防治提供了有价值的分子生物学依据。The genetic diversity of eight characteristic geographical populations of Plutella xylostella from China was investigated with ISSR method using fifteen selective primers in this study in order to clarify the genetic diversity of different geographic populations of P. xylostella. The results showed that total 395 ISSR loci, of which 89. 11% are polymorphic, were obtained, and the characteristic ISSR pattern for each P. xylostella individual was detected. The proportion of polymorphic bands （P）, Shannon＇ s index （I） and Nei＇ s gene diversity index （H） of P. xylostella displayed a high degree of genetic diversity both at the species level （ P = 89.11%, H = O. 2706, I = 0. 4286） and at the population level （ P = 88.80%, H = 0. 2759, I = 0. 4349 ）. The genetic diversity of P. xylostella was higher in areas of southern China （ Yunnan and Hubei） than in areas of northern China （ Beijing, Tianjin, Shandong, Heilongjiang, and Gansu）. The intra-population genetic variation was the highest in Nankou of Beijing, and the lowest in Haikou of Hainan and Lanzhou of Gansu. In additional, 5. 66% genetic variations are from intra- population and 94.34% from inter-population （Nm = 8. 3399）. No obvious genetic differentiation exists among the eight characteristic geographical populations of P. xylostella in this study. The results provide a valuable basis for the pesticide resistance control and the integrated management of the field population of P. xylostella.

关 键 词：小菜蛾 地理种群 遗传多样性 ISSR 聚类分析 

分 类 号：Q968[生物学—昆虫学]