海藻玉壶汤及其拆方对大鼠碘缺乏致甲状腺肿的干预作用  

Intervention Effect of Haizao Yuhu Decoction and Its Decomposed Recipes for Goiter Caused by Iodine Deficiency

作　　者：高天舒[1] 齐腾澈[1] 

机构地区：[1]辽宁中医药大学附属医院内分泌科,辽宁省沈阳市北陵大街33号110032

出　　处：《中医杂志》2012年第19期1671-1676,共6页Journal of Traditional Chinese Medicine

基　　金：教育部科技发展中心高等学校博士学科点专项科研基金资助项目(20092133110004);辽宁省教育厅高等学校科研项目资助项目(2009A492);辽宁省高等学校优秀人才支持计划资助项目(LR201026)

摘　　要：目的从抗氧化应激角度比较海藻玉壶汤及其拆方对碘缺乏甲状腺肿的干预机制。方法选4周龄Wistar大鼠150只,随机选取30只为正常对照组,其余120只建立碘缺乏动物甲状腺肿模型后随机分为模型对照组和海藻玉壶汤原方组(原方组)、拆方1组、拆方2组,每组30只。在造模成功0周和8周后处死大鼠。测定各组大鼠尿碘含量(MUI);甲状腺功能[包括血清促甲状腺激素(TSH)、血清总三碘甲状腺原氨酸(TT3)、总四碘甲状腺原氨酸(TT4)]及相对重量;光镜下定量观察甲状腺形态及电镜下观察甲状腺细胞超微结构;测定血清谷胱甘肽过氧化酶(GSH-Px)、黄嘌呤氧化酶(XOD)和超氧化物歧化酶(SOD)的活力及丙二醛(MDA)和过氧化氢(H2O2)的含量;检测大鼠甲状腺4-羟基壬烯醛(4-HNE)、过氧化物氧化还原酶5(PRDX5)及其蛋白的表达。结果给药8周后,模型对照组和拆方1组MUI减低,原方组和拆方2组MUI增高。3个给药组TSH值较模型对照组显著下降(P<0.05)。TT3值原方组较正常对照组显著减低(P<0.01),拆方1组较模型对照组和原方组均显著增高(P<0.05)。TT4值原方组和拆方2组显著高于正常对照组和模型对照组(P<0.05或P<0.01),拆方1组明显低于原方组(P<0.05)。甲状腺相对重量原方组和拆方2组明显较模型对照组减低(P<0.01),拆方1组显著高于正常对照组(P<0.05)。原方组SOD活力较正常对照组和拆方2组明显增高(P<0.05)。GSH-Px活力原方和拆方1组较拆方2组明显增高(P<0.05)。原方组H2O2含量较正常对照组明显增高(P<0.05)。MDA含量拆方1组显著高于正常对照组(P<0.05)。各给药组XOD活力较正常对照组和模型对照组均明显增高(P<0.01),其中拆方1组明显高于原方和拆方2组(P<0.01)。甲状腺4-HNE表达原方组明显低于其他各组(P<0.05)。原方组甲状腺PRDX5蛋白较正常对照组有明显增高(P<0.01)。结论海藻玉壶汤原方较其拆方抗氧化能力更强,可使甲状腺Objective To compare the intervention mechanism of Haizao Yuhu Decoction （Sargassum Jade Flask Decoction） and its decomposed recipes for goiter caused by iodine deficiency from anti-oxidative stress. Methods Totally 150 4-week-old Wistar rats were selected. Thirty randomly-selected rats were taken into normal control group, and others were used to make the models of goiter caused by iodine deficiency, the rats were devided into model control （MC） group, Haizao Yuhu Decoction group （HZ group）, Haizao Yuhu Decoction decomposed recipe group A （HZA group） and Haizao Yuhu Decoction decomposed recipe group B （HZB group） with 30 in each group. The rats were killed after modeling and 8 weeks later respectively. The median of urinary iodine （MUD, thyroid stimulating hormone （TSH）, total triiodothyronine （TT3） and total thyroxine （TT4） was measured. The quantitative observation of thyroid morphology was conducted under optical microscope and the uhrastruc- ture of thyroid cells was observed under electron microscopy. The contents of serum glutathione peroxidase （GSH-Px）, xan- thineoxidase （XOD）, superoxide dismutase （SOD）, malondialdehyde （MDA） and hydrogen peroxide （H202） were detected. The expression of 4-hydroxynonenal （4-HNE） in the thyroid was detected by the immunohistochemistry method. The expres- sion of peroxide oxidation reduction enzyme5 （PRDX5） in the thyroid was detected by the Western blotting and immunohisto- chemistry method. Results After 8 weeks of treatment, MUI was decreased in the MC group and HZA group, while MUI was increased in the HZ group and HZB group. TSH value was significantly decreased in the three treated groups as compared with the MC group （P^0.05）. TT3 value in the HZ group was obviously decreased as compared with the NC group （P% 0.01）. TT3 value in the HZA group was obviously increased as compared with the MC group and HZ group （P%0.05）. TT4 value in the HZ group and HZB group was significantly higher than that in

关 键 词：氧化应激 甲状腺肿 海藻玉壶汤 富碘中药 

分 类 号：R285.5[医药卫生—中药学]