BMP4/Smad4在C57BL/6小鼠内耳前庭发育不同阶段的表达  

Expression of BMP4/Smad4 in C57BL/6 Mice Vestibule at Different Developmental Stages

作　　者：邓安春[1] 杨仕明[2] 黄德亮[2] 孙建和[2] 

机构地区：[1]第三军医大学新桥医院耳鼻咽喉科,重庆400037 [2]解放军总医院耳鼻咽喉头颈外科解放军耳鼻咽喉研究所

出　　处：《听力学及言语疾病杂志》2012年第5期462-466,共5页Journal of Audiology and Speech Pathology

基　　金：国家自然科学基金重点项目(30730040)和面上项目(30571017、30871398);重庆市回国人员启动基金资助项目(CSTC,2010BB5032);第三军医大学回国人员启动基金资助项目(2010XHG05)

摘　　要：目的探讨骨形态形成蛋白4(bone morphogenetic protein 4,BMP4)和Smad4蛋白(drosophila mothers against decapentaplegic protein 4)在C57BL/6小鼠内耳前庭发育不同阶段的表达。方法选择从胚胎第10天(E10)到胚胎第20天(E20)每天的孕鼠各两只(共22只),取E10～E17的胚胎头、E18～E20的胚胎内耳,通过冰冻连续切片、免疫组化或免疫荧光染色,观察小鼠胚胎内耳前庭发育过程中BMP4/Smad4的表达情况。结果 BMP4从E10开始,在间充质浓聚前就有表达;间充质一开始浓聚,BMP4在其中就有较丰富的表达;早期主要表达在前庭囊及前庭囊周围的间充质,在胚胎发育的后期,其在软骨囊及前庭上皮里有广泛阳性表达;Smad4在小鼠内耳前庭的表达情况与BMP4不一致,E10到E12的小鼠内耳Smad4表达阴性,E15时各个壶腹嵴、囊斑里才有较明显的Smad4表达,而内耳周围软骨囊到E16时才有明显的Smad4表达。结论 BMP4与小鼠内耳前庭形态形成及毛细胞分化发育、功能形成有关;Smad4与BMP4的表达时空特征不同,Smad4可能主要参与了小鼠内耳前庭发育后期的形态塑形和功能发育。Objective To explore the expression of BMP4/Smad4 in C57BL/6 mice vestibule at different developmental stages.Methods Using embryonic head from E10 to E17 old mice and embryonic inner ear from E18 to E20 old mice,the expression of BMP4/Smad4 in C57BL/6 mice vestibule at different developmental stages were investigated through frozen serial sectioning,immunohistochemistry and immunofluorescence staning.Results There was BMP4 expression at E10 in epithelium of otic capsule.Later,BMP4 expressions were affluent in condensed mesenchyme,vestibular cartilage capsule and sense epithelium.The expression pattern of Smad4 in vestibule was different from that of BMP4.It was not conspicuous until E15 that Smad4 expressed in macula and crista ampullaris and until E16 in periotic cartilage capsule.Conclusion BMP4 is involved in vestibular morphogenesis,hair cell differentiation and function acquiring.The expression temporal spatial pattern of Smad4 in vestibule during each development stage is different from that of BMP4.Smad4 may play a role in morphous modification and functional development during late stage of vestibular development in mice inner ear.

关 键 词：小鼠 前庭 BMP4/Smad4 

分 类 号：R764.35[医药卫生—耳鼻咽喉科]