广西桂北地区HBV感染者不同免疫状态HBV基因型分布研究  

Genotypes Distribution among Hepatitis B Virus Infectied Patients with Different Immune Statuses in Guangxi North Region

作　　者：杨丽莎[1] 吴淋玲[1] 蒋冬香[1] 王绩业[2] 黄亚琴[1] 

机构地区：[1]桂林医学院附属医院,桂林41001 [2]桂林医学院附属传染病院,桂林41001

出　　处：《病毒学报》2012年第5期536-540,共5页Chinese Journal of Virology

基　　金：国家自然科学基金资助项目(批准文号30760294);广西壮族自治区卫生厅重点科研课题(重200978)

摘　　要：为了了解广西桂北地区乙型肝炎病毒(Hepatitis B Virus,HBV)感染者基因型分布情况及探讨HBV感染不同免疫状态与基因型的关系。将HBV感染者按免疫耐受、免疫清除(应答)和免疫不全(病毒残留)三种免疫状态分类,各选150例,共450例,运用实时荧光定量PCR法检测HBV感染三种不同免疫状态者的HBV基因型。450例中B型为323例、C型为94例、B+C混合型为23例和非B非C型为10例;HBV感染三种免疫状态均B型占多数,分别为70.0%、78.0%和67.33%,不同免疫状态基因型构成比差异无统计学意义;免疫状态与基因型相关性无统计学意义;B型HBV-DNA载量高于C型,各组中年龄≥30岁者C型显著多于<30岁者,差异有统计学意义;各基因型间丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总胆红素(TBil)阳性率差别无统计学意义;男女基因型分布差异无统计学意义。结果表明,广西桂北地区HBV基因型以B型为主,C型占部分比例,少量B+C混合型,偶有未能分型;HBV感染免疫耐受、免疫清除(应答)和免疫不全(病毒残留)三种免疫状态均B型占多数,慢性HBV感染免疫状态与HBV基因型相关性无统计学意义。In order to find out the distribution of Genotype of those people infected with HBV（hepatitis B virus ）from north Guangxi and the relationship between different immune status of HBV infected people and their genotypes, the HBV infected people are classified into three types according to immune tolerance, immune clearance （ response ） and immune incompetence （residues） . 150 cases from each type, a total of 450 cases are chosen to be tested with real time fluorescence quantitative PCR assay for detection of HBV infection in three kinds of different immune state of the HBV genotype. In the 450 cases, 323 cases belong to type B, 94 cases belong to type B, 23 cases belong to mixed type B＋C and 10 cases belong to none B and none C type. Type B are the majority in all the three HBV immune status, made up to 70%, 78%, 67.33% of each type. The different immune state genotype proportion difference don＇t have statisti- cal significance; immune state and genotypic correlation isn＇t statistically significant; type B HBV-DNA load is higher than that of type C, groups of persons aged 30 years or older with type C are significantly higher than that of 〈 30 years of age, the difference was statistically significant; among the genotypes of alanine aminotransferase （ ALT ）, aspartate aminotransferase （ AST ）, total bilirubin （ TBil ） positive rate showed no significant difference between male and female; there was no significant difference in geno- type distribution. The results show that, in North Guangxi HBV genotypes B, C accounts for the proportion, a small amount of B＋C hybrid, occasionally fails to type HBV infection; among immune tolerance, immune clearance （ response ） and immune incompetence （ residues ） type B are in majority in these three kinds of immune state, chronic HBV infection immunity with the HBV genotype correlations were not sta- tistically significant.

关 键 词：乙型肝炎病毒 免疫耐受 免疫清除 免疫不全 HBV基因型 

分 类 号：R373.2[医药卫生—病原生物学]