CDCA在C57BL/6小鼠中上调肝脏成纤维细胞生长因子21表达及其效应  

Chenodesoxycholic acid up-regulates fibroblast growth factor 21 and its effect on C57BL/6 mice

作　　者：刘小花[1] 王永超[2] 李良鹏[2] 彭家和[2] 江渝[2] 刘智敏[1] 

机构地区：[1]重庆医科大学基础医学院生物化学与分子生物学教研室,分子医学与肿瘤研究中心,重庆400016 [2]第三军医大学基础部生物化学与分子生物学教研室,重庆400038

出　　处：《第三军医大学学报》2012年第18期1831-1834,共4页Journal of Third Military Medical University

基　　金：国家自然科学基金(81070228)~~

摘　　要：目的研究法尼酯X受体(farnesoid X receptor,FXR)的激活对C57BL/6小鼠肝脏中纤维细胞生长因子21(fibroblast growth factor 21,FGF21)基因表达的影响。方法将18只3～4周龄C57BL/6小鼠随机分为3组,分别使用不同浓度鹅脱氧胆酸(chenodeoxy cholic acid,CDCA,0、10 mg/kg和50 mg/kg)给小鼠灌胃7 d,处死后分别提取小鼠肝脏总RNA和总蛋白,采用RT-PCR法和Western blot法检测各组小鼠FGF21RNA和蛋白表达水平的变化;同时用酶法测定检测小鼠血甘油三酯(TG)、胆固醇(TC)及血糖(GLU)变化。结果 C57BL/6小鼠肝脏中FGF21 RNA和蛋白的表达水平呈CDCA剂量依赖型增高;小鼠血脂及血糖测定结果显示:TG,TC和血糖随CDCA浓度的升高而下降。结论 CDCA可上调C57BL/6小鼠肝脏中FGF21的表达并导致血TG、TC及GLU下降。Objective To determine the activation of the farnesoid X receptor （FXR） on the expression of fibroblast growth factor 21 （FGF21） in C57BL/6 mice. Methods A total of 18 male C57BL/6 mice （3 to 4 weeks old） were randomly divided into 3 groups and treated with FXR agonist, chenodesoxycholic acid （CDCA） at different doses of 0, 10, and 50 mg/kg respectively for a week. After the treatment, the mice was sacrificed for their liver tissue. RT-PCR and Western blotting were performed to test the expression of FGF21 at mRNA and protein levels in the liver tissue. Blood triglycerides （TG） , cholesterol （TC） and blood glucose （GLU） were measured by enzymatic determination test. Results FXR agonist CDCA up-regulated the FGF21 at mRNA and protein levels in a dose-dependent manner in C57BL/6 liver tissue after treatment for a week. While, blood concentrations of TG, TC and GLU were decreased with the increase of CDCA doses. Conclusion CDCA up-regulates FGF21 expression in C57/BL6 mice liver and results in a decrease in plasma TG, TC and blood glucose.

关 键 词：法尼酯X受体 成纤维细胞生长因子21 鹅脱氧胆酸 血脂 血糖 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学] R329.26[医药卫生—基础医学]