半胱氨酸蛋白酶-8和P53在慢性砷中毒大鼠肾近端小管表达的研究  

Expression of cysteine caspase-8 and P53 in renal proximal tubular epithelial cell of chronic arsenic poisoning rats

作　　者：钱立全[1] 李远慧[1] 孔祥照[1] 金婷婷[1] 李娜[1] 

机构地区：[1]贵阳医学院组织胚胎学教研室,550004

出　　处：《中国地方病学杂志》2012年第5期531-533,共3页Chinese Jouranl of Endemiology

基　　金：贵州省科技攻关项目（黔科合S字1059号）

摘　　要：目的探讨慢性砷中毒大鼠肾近端小管细胞半胱氨酸蛋白酶-8（caspase-8）和P53表达致肾脏损伤的分子机制。方法60只SD大鼠分为对照组，低、高剂量组，每组20只，高、低剂量组分别给予三氧化二砷（As2O3）10．0、0．4mg／kg水溶液自由饮用，对照组自由饮用蒸馏水。分笼4个月，测定血砷、尿砷，取肾脏标本，组织学技术HE染色，免疫组织化学（SABC）法检测大鼠肾小管上皮细胞caspase-8、P53免疫阳性细胞，并进行计数和图像分析研究。结果对照组，低、高剂量组肾近端小管上皮caspase-8阳性细胞计数[（3．33±1．32）、（31．14±8．02）、（46．50±7．20）个／视野]依次增加（P均〈0．05），平均灰度值（151．34±6．40、133．58±4．63、128．34±16．28）依次降低（P均〈0．05），P53阳性细胞计数[（3．17±1．59）、（26．29±4．23）、（47．00±6．22）个／视野]依次增加（P均〈0．05），平均灰度值（142．54±8．06、121．48±5．68、101．89±6．35）依次降低（P均〈0．05）。结论caspase-8、P53阳性细胞增多是砷中毒致肾脏损伤的分子机制之一。Objective To study the molecular mechanism of renal injury of chronic arsenic poisoning rats induced by the expression of cysteine caspase-8 and P53 in renal proximal tubular epithelial cells. Methods Sixty healthy SD rats were divided into three groups, high-, low-dose group, and control group, n = 20 in each group. The rats in high and low dose groups were treated with As203 through drinking water, 10.0 and 0.4 mg/kg, respectively. The control rats were given distilled water. Four months later, serum and urinary arsenic level was determined, and kidney specimens were taken. The expression of cysteine caspase-8 and P53 in renal proximal tubular epithelial cells was detected by histological technique-HE staining and SABC immunohistochemistry. In addition, cell number counting and image analyses were used in the study. Results The number of caspase-8 positive cells of renal proximal tubule in control group, low- and high-dose group was 3.33 ± 1.32, 31.14 ± 8.02 and 46.50 ± 7.20 cell number/visual fields, respectively, which was increased with dose increasing（all P 〈 0.05）; the average gray value was 151.34 ± 6.40, 133.58 ± 4.63 and 128.34 ± 16.28, respectively, decreased with dose increasing（all P 〈 0.05）. The number of P53 positive cells was 3.17 ± 1.59, 26.29 ± 4.23 and 47.00 ± 6.22 cell number/visual fields, respectively, increased with dose increasing （all P 〈 0.05 ） ; the average gray value was 142.54 ± 8.06, 121.48 ± 5.68 and 101.89 ± 6.35, respectively, decreased with dose increasing （all P 〈 0.05）. Conclusion The increase of caspase-8 and P53 positive cells is one of the molecular mechanisms of renal injury induced by arsenic poisoning.

关 键 词：砷中毒 大鼠 肾近端小管 半胱氨酸蛋白酶-8 P53 

分 类 号：R692[医药卫生—泌尿科学]