反透析法测定阿奇霉素囊泡的包封率  

Determination of Entrapment Efficiency of Azithromycin Niosomes by Reverse Dialysis Method

作　　者：钟萌[1] 尹华峰[1] 滕永真[1] 吴建勇[1] 张景勍[1] 

机构地区：[1]重庆医科大学药物高校工程研究中心和生物化学与分子药理学重点实验室,重庆市渝中区医学院路1号400016

出　　处：《光谱实验室》2012年第5期2940-2943,共4页Chinese Journal of Spectroscopy Laboratory

基　　金：教育部博士点基金资助项目(20095503120008);重庆市教育委员会资助项目(首批高等学校优秀人才项目;KJ090308)

摘　　要：建立阿奇霉素囊泡的包封率测定方法。以紫外分光光度法为分析手段,采用反透析法测定阿奇霉素囊泡的包封率。反透析法透析平衡时间为8h,游离药物的绝对回收率符合要求。该方法测得自制阿奇霉素囊泡的平均包封率为92.51%±0.56%,10h内没有渗漏,方法重现性好。该法操作准确、简便,可用于测定阿奇霉素囊泡的包封率。The method for determination of entrapment efficiency of azithromycin niosomes was established.The entrapment efficiency of azithromycin niosomes was determined by reverse dialysis method with UV spectrophotometry.The balance time of reverse dialysis method was 8h,and the absolute recovery of free drug agreed with the requirement.The average entrapment efficiency of azithromycin niosomes was 92.51%±0.56%.The repeatability of the method is good with no leakage in 10h.The method is accurate and simple,that can be applied to determination of entrapment efficiency of azithromycin niosomes.

关 键 词：反透析法 阿奇霉素囊泡 包封率 

分 类 号：O657.32[理学—分析化学]