大鼠肝微粒体中盐酸度洛西汀的RP-HPLC测定  

Determination of Duloxetine Hydrochloride in Rat Liver Microsomes by RP-HPLC

作　　者：刘丹[1] 邹黎[1] 雷琪[1] 黄开顺[1] 朱链链[1] 陈志琼[1] 

机构地区：[1]重庆医科大学药物分析教研室,重庆市渝中区医学院路1号400016

出　　处：《光谱实验室》2012年第5期2948-2951,共4页Chinese Journal of Spectroscopy Laboratory

摘　　要：建立大鼠肝微粒体中盐酸度洛西汀的RP-HPLC测定方法,为其体外代谢研究奠定基础。盐酸度洛西汀与大鼠肝微粒体共孵育后,用甲醇沉淀蛋白。离心上清液,采用HP1100色谱系统,以甲醇-0.025mol.L-1磷酸二氢钾缓冲盐(62∶38,V/V,pH 3.5)为流动相,流速0.9mL.min-1,进样量20μL,经Hedera ODS-2(4.6mm×250mm,5μm)色谱柱分离,检测波长290nm。盐酸度洛西汀在0.4—16μg.mL-1范围内线性关系良好(r=0.9992),检测限为40ng.mL-1,定量限为0.2μg.mL-1,方法回收率在94.4%—105.0%之间,日内、日间精密度分别<5%和<10%(n=5)。本方法较为快速,灵敏,准确,可用于盐酸度洛西汀在大鼠肝微粒体中的体外代谢研究。The method for the determination of duluoxitine hydrochloride in rat liver microsomes by RP-HPLC was established,that provided the basis for supersession study in vitro.After duluoxitine hydrochloride and rat liver microsomes were carried out to incubate,protein was precipitated by methanol.The liquid supernatant was centrifuged,then was carried out by HP1100 chromatographic system with methanol-0.025mol·L^-1 monopotassium phosphate buffer salt（62∶38,V/V,pH 3.5） as mobile phase at the flow rate of 0.9mL·min-1,and injection amount was 20μL,and the compound was separated by Hedera ODS-2 column（4.6mm×250mm,5μm）,and detection wavelength was 290nm.There was good linear relationship for duluoxitine hydrochloride in the range of 0.4—16μg·mL^-1 with correlation coefficient of 0.9992,and the detection limit was 40ng·mL^-1,and quantitation limit was 0.2μg·mL^-1,and the recoveries of the method was between 94.4% and 105.0%,and precisions of inter-day and intra-day were respectively less than 5% and less than 10%（n=5）.The method is rapid,sensitive and accurate,and can be applied to investigating the supersession of duluoxitine hydrochloride in rat liver microsome in vitro.

关 键 词：盐酸度洛西汀 高效液相色谱法 大鼠肝微粒体 体外代谢 

分 类 号：O657.72[理学—分析化学]