超临界二氧化碳提取沙芥总黄酮的工艺  

Extraction of Flavonoids from Pugionium cornutum(L.) Gaerth by Supercritical CO_2 Fluid

作　　者：温俊峰[1] 刘步明[1] 王高祥[1] 

机构地区：[1]榆林学院化学与化工学院,陕西省榆林市西沙崇文路2号719000

出　　处：《光谱实验室》2012年第5期3130-3134,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：榆林市科技计划项目(Ng09-1-05);榆林学院院级科研项目(09-YK30)

摘　　要：采用超临界CO2萃取法提取沙芥中总黄酮,通过单因素试验和正交试验考查提取温度、提取压力、提取时间和夹带剂流速对沙芥黄酮提取结果的影响,确定沙芥总黄酮超临界CO2提取工艺的最佳条件。结果表明,最佳参数组合为:温度35℃,压力30MPa,提取时间2.5h,80%乙醇流速0.2mL/min,在此工艺条件下沙芥黄酮的提取率为9.20mg/g。Flavonoids compounds was extracted from Pugionium cornutum（L.） Gaerth by supercritical CO2 fluid extraction method.The single-factor and orthogonal design experiments were adopted to investigate the effects of extracting temperature,pressure,time and the ethanol flow rate on the extraction efficiency.The results showed the optimal extraction conditions were as follows：extraction temperature of 35℃,extraction pressure of 30MPa,80% ethanol flow rate of 0.2mL/min,extraction time of 2.5h.Under the optimal extraction conditions,the extraction efficiency of total flavonoids was as high as 9.20mg/g.

关 键 词：沙芥 黄酮 超临界CO2提取 正交试验 

分 类 号：O657.32[理学—分析化学]