光谱法研究橙黄Ⅴ与牛血清白蛋白的相互作用  

Interaction of Bovine Serum Albumin with Orange Ⅳ by Spectrometry

作　　者：王欢[1] 王晓玲[1] 刘建波[1] 

机构地区：[1]咸阳师范学院化学与化工学院

出　　处：《光谱实验室》2012年第5期3148-3152,共5页Chinese Journal of Spectroscopy Laboratory

基　　金：陕西省教育厅项目(11JK0580)

摘　　要：在pH=7.40的缓冲条件下,采用荧光光谱法和紫外-可见分光光度法研究了橙黄与牛血清白蛋白(BSA)的相互作用,研究发现橙黄对BSA有较强的荧光猝灭作用,用Stern-Volmer和Lineweaver-Burk方程分别处理实验数据,得到了猝灭常数Ksv、Kq、热力学参数(ΔHθ,ΔSθ,ΔGθ)和结合位点数等,证明二者主要靠范德华作用和氢键作用,同时用同步荧光光谱法探讨了橙黄对BSA构象的影响。The interaction between orange Ⅳ and bovine serum albumin（BSA） was investigated by fluorescence spectrometry and UV-Vis spectrometry under the buffer condition with pH of 7.40.The quenching mechanism was analyzed referring to orange Ⅳ against the fluorescence of BSA.The experimental data was deal with by Stem-Volmer equation and Lineweaver-Burk equation,then the quenching constants Ksv,Kq,the thermodynamic parameters（ΔH^θ,ΔS^θ,ΔG^θ） and the number of binding sites were obtained,and the main forces between orange Ⅳand BSA were Van der Waals and hydrogen bind,meanwhile,the influence of orange Ⅳ on BSA was investigated by synchronous fluorescence spectrometry.

关 键 词：橙黄Ⅳ 牛血清白蛋白 荧光光谱法 紫外可见分光光度法 

分 类 号：O657.32[理学—分析化学]