颅内出血早产儿血浆血小板活化因子水平及其乙酰水解酶活性的变化及其意义  

Changes of Blood Plasma Platelet-Activating Factor and Platelet-Activating Factor-Acetylhydrolase in Neonatal Intracranial Hemorrhage and Their Significances

作　　者：张茜[1] 程欣茹[1] 徐淑玲[2] 罗成汉[1] 时赞扬[1] 盛光耀[1] 

机构地区：[1]郑州大学第一附属医院儿科,郑州450052 [2]郑州市妇幼保健院儿科,郑州450012

出　　处：《实用儿科临床杂志》2012年第18期1436-1438,共3页Journal of Applied Clinical Pediatrics

基　　金：河南省科技厅基金(083SGYS33263-5)

摘　　要：目的探讨新生儿血浆血小板活化因子(PAF)水平及其乙酰水解酶(PAF-AH)活性与颅内出血(ICH)的相关性。方法选取48例ICH早产儿为出血组,48例非ICH早产儿为非出血组(根据头颅彩超结果进行诊断)。采集2组脐血、出生3 d及7 d外周静脉血,应用ELISA法检测其血浆PAF水平及PAF-AH活性,并动态观察其变化,不同组间均数比较采用单因素方差分析及两两比较,出血程度与PAF水平及PAF-AH活性行直线相关分析。结果 1.出血组血浆PAF水平均高于同期非出血组(F=219.358,P<0.01),2组脐血及出生3 d PAF水平比较差异均有统计学意义(Pa<0.01),7 d PAF水平比较差异无统计学意义(P>0.01)。出血组组内比较差异有统计学意义(F=280.135,P<0.01),颅内出血活动期(出生3 d)水平最高、出血稳定期(7 d)水平降低,二者比较差异有统计学意义(P<0.01)。2.出血组PAF-AH活性均低于非出血组(F=279.376,P<0.01),2组脐血及出生3 d水平两两比较差异均有统计学意义(Pa<0.01)、7 d时PAF-AH水平比较差异无统计学意义(P>0.01)。出血组组内比较差异有统计学意义(F=165.368,P<0.01),ICH活动期PAF-AH活性较低、稳定期PAF-AH活性升高。结论 PAF-AH活性降低及PAF水平升高为早产儿ICH的重要因素之一,而ICH的发生及转归与PAF水平、PAF-AH活性的变化有密切相关性。Objective To explore the role of platelet - activating factor （PAF） and PAF - acetylhydrolase （ PAF - AH ） in neonates with intracranial hemorrhage（1CH）. Methods Forty - eight neonates with ICH were selected as ICH group and 48 cases of non - ICH patients were selected as non - ICH group. The umbilical cord blood and peripheral blood samples of neonates on day 3, day 7 of life were collected. The blood plasma PAF levels and PAF - AH activity were detected by enzyme linked immunosorbent assay and the dynamic changes were observed. Plasma PAF levels and PAF - AH activity between different groups were compared by ANOVA - Pairwise comparisons. The correlations between degree of ICH and plasma PAF levels and PAF - AH activity were analyzed. Results l. Plasma PAF levels in umbilical cord blood and peripheral blood on day 3, day 7 of neonates in ICI-I group were higher than those in non -ICH group（ F = 219. 358, P 〈 0. 01 ） , the differences were significant in umbilical cord blood and peripheral blood on day 3 （ Pa 〈 0.01 ）, but not on day 7 （ P 〉 0. O1 ）. There had significant difference in ICH group （ F = 280. 135, P 〈 0.01 ）, plasma PAF level was highest in active period of ICH （ on day 3 ）, and decreased in stable period （ on day 7）, the difference was significant（ P 〈 0.01 ）. 2. Plasma PAF - AH activity in umbilical cord blood and peripheral blood on day 3, day 7 of neonates in ICH group were lower than those in non - ICH group （ F = 279. 376, P 〈 0.01 ）, this differences were significant in umbilical cord blood and peripheral blood on day 3 （ Pa 〈 0.01 ）, but not on day 7 （ P 〉 0.01 ）. There had significant difference in ICH group （ F = 165. 368, P 〈 0.01 ）. Plasma PAF - AH activity was lower in active period of ICH, and elevated in sta- ble period （P 〈 0.01 ）. Conclusions High PAF level and low PAF - AH activity may be one of the risk factor of ICH in neonates. Furthermore, the process of ICH was closely related with PAF level and PA

关 键 词：血小板活化因子 血小板活化因子乙酰水解酶 早产儿 颅内出血 

分 类 号：R722.6[医药卫生—儿科]