Peyer小结在IgA肾病发病机制中的作用  

Role of Peyer′s Patches in Pathogenesis of IgA Nephropathy

作　　者：邹敏书[1,2] 周建华[1] 尹晓玲[1] 胡长霞[1] 杨凤杰[1] 王嘉[1] 陈瑜[1] 张瑜[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院儿科,武汉430030 [2]广州军区武汉总医院儿科,武汉430070

出　　处：《实用儿科临床杂志》2012年第17期1313-1315,共3页Journal of Applied Clinical Pediatrics

基　　金：国家自然科学基金(81070557)

摘　　要：目的探讨Peyer小结在IgA肾病(IgAN)发病中的作用。方法雄性BALB/C小鼠予牛血清清蛋白、葡萄球菌肠毒素B诱导IgAN模型,另选40只正常雄性BALB/C小鼠作为对照组。第12周免疫荧光检测其肾组织IgA沉积,测定其血清总蛋白(TP)、清蛋白(Alb)、BUN、Cr、半胱氨酸蛋白酶抑制剂C(Cys C)。荧光定量反转录(RT)-PCR检测Peyer小结IL-21、转化生长因子-β1(TGF-β1)mRNA的表达。Western blot测定Peyer小结IL-21、B细胞淋巴瘤因子-6(Bcl-6)、B淋巴细胞诱导成熟蛋白-1(Blimp-1)蛋白表达。结果 1.IgAN组和对照组第0周、第6周、第12周24 h尿蛋白定量分别为(0.61±0.26)mg vs(0.67±0.22)mg、(3.61±0.75)mg vs(0.77±0.27)mg、(4.15±0.82)mg vs(2.09±0.51)mg,IgAN组第6周、第12周24 h尿蛋白均高于对照组(Pa<0.01)。2.IgAN组和对照组IL-21、TGF-β1 mRNA表达相对值分别为1.67±0.13 vs 1.48±0.13、1.21±0.09 vs 1.10±0.10,与对照组相比,IgAN组IL-21、TGF-β1 mRNA表达均增加,差异均有统计学意义(Pa<0.05)。3.Western blot显示IgAN组和对照组IL-21、Bcl-6、Blimp-1蛋白表达相对值分别为0.67±0.21 vs 0.45±0.10、0.34±0.21 vs 0.60±0.19、1.03±0.07 vs 0.67±0.07,与对照组相比,IgAN组IL-21、Blimp-1蛋白表达均显著增高,差异均有统计学意义(Pa<0.05),Bcl-6蛋白表达降低,差异有统计学意义(P<0.05)。结论 Peyer小结有促进B淋巴细胞向分泌IgA浆母细胞分化的微环境,参与IgAN的发病。Objective To investigate the role of Peyer′s patches in pathogenesis of IgA nephropathy（IgAN）. Methods The nephropathy model was induced by means of bovine serum albumin and staphylococcal enterotoxin B injection in male BALB/C mice.And 40 male BALB/C mice were assigned as control group.At 12 week,the deposition of IgA was examined in renal tissues by immunofluorescence,and serum levels of total protein（TP）,albumin（Alb）,urea nitrogen（BUN）,creatinine（Cr）,and Cystatin C（Cys C）were detected.Interleukin-21（IL-21） and transforming growth factor-β1（TGF-β1） mRNA were detected by fluorescent quantitative reverse transcription-polymerase chain reaction in Peyer′s patches.The protein expressions of IL-21,B cell lymphoma-6（Bcl-6） and B lymphocyte-induced ma-turation protein-1（Blimp-1） in Peyer′s patches were measured by Western blot,respectively. Results 1.Twenty-four-hour urinary protein at 0 week,6 week,12 week in the IgAN group and control group were（0.61±0.26） mg vs（0.67±0.22） mg,（3.61±0.75） mg vs（0.77±0.27） mg,（4.15±0.82） mg vs（2.09±0.51） mg,respectively.Twenty-four-hour urinary protein at 6 week,12 week in IgAN group were significantly increased compared with those in control group（Pa〈0.01）.2.The mRNA expressions of IL-21 and TGF-β1 in IgAN group and control group were 1.67±0.13 vs 1.48±0.13,1.21±0.09 vs 1.10±0.10,respectively.Compared with the control group,the mRNA expressions of IL-21 and TGF-β1 were significantly increased in IgAN group（Pa〈0.05）.3.The protein expressions of IL-21,Bcl-6 and Blimp-1 in IgAN group and control group were 0.67±0.21 vs 0.45±0.10,0.34±0.21 vs 0.60±0.19,1.03±0.07 vs 0.67±0.07,respectively.Compared with control group,the protein expressions of IL-21 and Blimp-1 were significantly increased（Pa 〈0.05）,while the protein expression of Bcl-6 was significantly decreased（P〈0.05）. Conclusions Peyer′s patches have the microenvironment which facilitates B cells differentiation into sec

关 键 词：Peyer小结 IGA肾病 白细胞介素-21 

分 类 号：R692.3[医药卫生—泌尿科学]