UPLC法同时测定黔产隔山消药材中3个C_(21)甾体酯苷的含量  

Simultaneous determination of three C_(21) pregnane glycosides in Cynanchum auriculatum by UPLC

作　　者：杨庆雄[1] 李松林[1] 张婕[2] 葛永昌[1] 严晓琳[1] 

机构地区：[1]贵州师范大学化学与材料科学学院,贵阳550001 [2]贵州省烟草科学研究所,贵阳550081

出　　处：《药物分析杂志》2012年第9期1525-1528,共4页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(30760032;31160076);贵州省中药现代化(黔科合社字[2008]5022号);贵州省科技基金(黔科合J字[2007]2145号);贵州省优秀科技教育人才省长专项基金(黔省专合字(2007)34号)

摘　　要：目的:建立同时测定民族药材隔山消中3个C21甾体酯苷(白首乌新苷F、G和H)的UPLC分析法。方法:样品经甲醇超声提取后,用UPLC-PDA进行分析检测;采用Waters Acquity UPLC系统,ACQUITY UPLC BEH C18(2.1 mm×100 mm,1.7μm)色谱柱,流动相为乙腈-水,梯度洗脱,流速为0.4 mL.min-1,检测波长220 nm,柱温30℃,进样量2μL。结果:白首乌新苷F、G和H 3个C21甾体酯苷在检测范围内均呈良好线性(r≥0.9995);平均加样回收率(n=3)在98.2%～104.1%之间,RSD均不大于1.1%。结论:UPLC分离效果及重复性好,且快速、简便,可作为隔山消药材质量控制的参考方法。Objective： To develop an ultra performance liquid chromatography （UPLC） method to simultaneously determine three pregnane glycosides（ cynanauriculosides F, G, H） in Cynanchum auriculatum. Methods： After the untrasonie -assisted extraction with methanol, the analyses were performed on a Waters UPLC system, equipped with a binary solvent manager, a sampler manager, a column compartment and a PDA detector. An ACQUITY UPLC BEH Cls column（2.1 mm ~100 ram, 1.7 pin） was used for all analyses. The investigated compounds were separa- ted with the mobile phases of water and acetonitrile in gradient elution, with a flow rate of 0.4 mL ~ min-l. The temperature of column was set at 30 ~C, and the injection volume was 2 p^L. The detection wavelength was set at 220 nm. Results： The investigated compounds including cynanauriculoside F, G and H had good linearity（ ≥0. 9995） over the tested ranges. The average recoveries（ n = 3） were 98.2% to 104.1% with RSDs ≤1.1%. Conclusion： The developed UPLC method is simple, sensitive and accurate with good repeatability, which is available for quality control of raw material of Cynanchum auriculatum.

关 键 词：超高效液相色谱 民族药材白首乌成分分析 隔山消(耳叶牛皮消) 甾体酯苷 白首乌新苷 

分 类 号：R917[医药卫生—药物分析学]