HPLC测定不同产地与不同部位千层塔中石杉碱甲和石杉碱乙含量  

Simultaneous determination of huperzine A and huperzine B in different parts of Huperzia serrata from different habitats by HPLC

作　　者：袁经权[1] 周小雷[1] 王硕[1] 闫志刚[1] 冯世鑫[1] 蒋娇梅[1] 朱大元[2] 马小军[1] 

机构地区：[1]广西药用植物研究所,南宁530023 [2]中国科学院上海药物研究所,上海201203

出　　处：《药物分析杂志》2012年第9期1541-1544,1549,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：广西应用基础研究专项(0991019);广西科技攻关项目(10100027-3与11107010-2-4);广西青年基金项目(2010GXNSFB013041);广西医疗卫生重点课题(2011031);南宁市科技攻关项目(201102076C)

摘　　要：目的:测定不同产地、不同部位千层塔药材中石杉碱甲和石杉碱乙的含量。方法:采用UItimate XB-C18(4.6 mm×250 mm,5μm)色谱柱,流动相为乙腈-甲醇-水(10∶55∶240),含0.32%磷酸和0.5%三乙胺,流速1.0 mL.min-1,检测波长309 nm,柱温25℃。结果:测得各地千层塔中石杉碱甲、石杉碱乙最高含量分别为0.0900%和0.0183%,各地石杉碱甲、石杉碱乙最低含量分别为0.0014%和0。结论:所测定的方法简便、可靠、快速,重复性好,结果稳定,适用于千层塔药材中石杉碱甲和石杉碱乙的同时快速含量测定,该研究可为千层塔最佳产地及最佳药用部位的确定提供参考。Objective： To develop an HPLC method for simultaneous determination of huperzine A （ Hup - A） and huperzine B （Hup -B） in different parts of Huperzia serrate from different habitats. Methods：The assay was per- formed on an Uhimate XB -C18（4.6 mm ×250mm,5 μm） column with the mobile phase of aeetonitrile- metha- nol - water （ 10 ： 55 ： 240 ） containing 0. 32 % phosphoric acid and 0.5 % triethylamine. The flow rate was 1.0 mL .min-1 ,the detection wavelength was set at 309 nm, and the column temperature was set at 25 ℃. Re- sults：The contents of huperzine A and huperzine B in Huperzia serrata collected at different habitats were evidently different. The maximum contents of huperzine A and huperzine B were 0. 0900% and 0. 0183% , respectively;the minimum contents of huperzine A and huperzine B were 0. 0014% and 0,respectively. Conclusions：The method is simple, accurate and repeatable, which provides a reliable way for evaluating the quality of Huperzia serrata by meas- uring huperzine A and huperzine B, as well as provide a reference for the determination of the best origin and the most rational part of the plant.

关 键 词：蛇足石杉 千层塔 石杉碱甲 石杉碱乙 高效液相色谱法 根、叶、茎成分分析 中药质量控制 不同产地品质评价 

分 类 号：R917[医药卫生—药物分析学]