多指标综合评判巴中产栀子的采收期和生长期  

Multi-index comprehensive evaluation on the reasonable harvesting and growing period of Fructus Gardeniae in Bazhong

作　　者：王子雯[1] 唐灿[1] 孙嘉婧[1] 王刚[1] 黄锐[1] 李希[2] 

机构地区：[1]泸州医学院药学院,泸州646000 [2]四川省中医药科学院中医研究所,成都610031

出　　处：《药物分析杂志》2012年第9期1554-1558,1563,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：四川省科技支撑计划项目-巴中产栀子品质与规范化种植研究(No.2010SZ0049);四川省科技支撑计划项目-巴中产栀子种质资源保护与评价研究(No.2011SZ0048);四川省教育厅重点科研项目-川浙赣产栀子中乌索酸含量的比较研究(No.2005A071)

摘　　要：目的:探索巴中产栀子的最佳采收期和生长期。方法:以HPLC-ELSD法测定巴中产栀子主要有效成分栀子苷、西红花苷Ⅰ、熊果酸含量,并采用t检验和Z-分综合评价法分析数据;测定栀子苷采用Kromasil C18键合硅胶柱(4.6 mm×250mm,5μm),流动相为乙腈-水(10∶90),流速1 mL.min-1,检测波长238 nm;测定西红花苷Ⅰ采用Dionex C18键合硅胶柱(4.6 mm×150 mm,5μm),流动相为甲醇-水(45∶55),流速1 mL.min-1,检测波长440 nm;测定熊果酸采用XDB C18色谱柱(4.6 mm×150 mm,5μm),以乙腈-水(81∶19)为流动相,流速1 mL.min-1,柱温30℃,采用蒸发光散射检测器,漂移管温度60℃。结果:t检验结果表明:巴中产30年生栀子西红花苷Ⅰ含量为红果(成熟期)>黄果(近成熟期)>绿果(未成熟期);栀子苷含量为绿果>黄果>红果;熊果酸含量为绿果>红果>黄果;30年生栀子红果中栀子苷、西红花苷Ⅰ和熊果酸含量均显著高于3年生红果。结论:以栀子苷、西红花苷Ⅰ、熊果酸多指标综合考察巴中产栀子的适宜采收期、生长期,对保证栀子的品质很有意义,也为后续研究提供了依据。Objective：To explore the optimal harvesting and growing period of Fructus Gardeniae in Bazhong; Methods： HPLC method was used to determine the contents of geniposide, crocin I , and ursolic acid. The data were analyzed by adopting t - test and Z score multi - index test. The content determination of geniposide was carried out on a Kromasil C18 （4.6 mm x 250 mm, 5 p,m） column with mobile phase of acetonitrile -wa- ter（ 10： 90） at a flow rate of 1 mL ~ rain-1, the UV detection wavelength was set at 238 nm. The content de- termination of crocin I was carried out on a Dionex Cl8 （4.6 mm x 150 ram, 5 txm） column with mobile phase of methanol- water（45： 55） at a flow rate of 1 mL ~ min i and UV detection wavelength at 440 nm. The content determination of ursolic acid was carried out on a XDB C18 （4.6 mm~ 150 mm, 5 ~m） heated to 25 ~C in the column compartment. The mobile phase was acetonitrile - water （81 ： 19） at a flow rate of 1 mL ~ rain-1 and drift tube of ELSD was heated to 60 ~C Results： Results of t - test showed that content of ero- cin I was increased from green fruit（ non - maturity） to red fruit（maturity） of 30 - year - old Fructus Gar- deniae ; content of geniposide was decreased from green fruit to red fruit ; content of ursolic acid was the highest in green fruit while it was the lowest in yellow fruit; the contents of geniposide, croein I , ursolic acid of 30 - year - old red fruit were significantly higher than those of 3 - year - old one. Conclusion ： It is of great signifi- cance to optimal the harvesting and growing period by investigation of geniposide, crocin I , and ursolic acid, which not only can ensure the quality of Fructus Gardenia from Bazhong, but also will provide an important hasis for the follow - up research.

关 键 词：巴中产栀子 栀子苷 西红花苷 熊果酸 中药采收期生长期成分分析 多指标综合评价 

分 类 号：R917[医药卫生—药物分析学]