枸骨叶中3类三萜皂苷类成分的ESI-MS裂解规律及其快速识别  

ESI-MS characterization and rapid identification of three types of triterpenoid saponins in Ilex cornuta

作　　者：张加余[1,2] 姚志容[1] 张凡[1] 屠鹏飞[1] 

机构地区：[1]北京大学药学院天然药物及仿生药物国家重点实验室,北京100191 [2]北京中医药大学中药学院,北京100102

出　　处：《药物分析杂志》2012年第9期1578-1582,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(30672608);国家重大新药创制专项(2009ZX311-004)

摘　　要：目的:研究枸骨叶中3类不同母核结构的三萜皂苷的ESI-MS裂解规律及其快速识别方法。方法:采用HPLC-ESI-MS技术研究3类三萜皂苷单体的ESI-MS(+)裂解规律,并采用该方法对药材中所含的3类成分进行快速识别。结果:3类三萜皂苷具有不同的ESI-MS(+)裂解特征;利用这些特征从枸骨叶提取物中快速筛选了29个三萜皂苷,其中10个可通过与对照品比对被准确鉴定。结论:利用HPLC-ESI-MS技术可以从复杂体系中快速筛选三萜皂苷类成分,为有目标的天然产物分离提供有效的方法。Objective ：To investigate the ESI - MS characterization and rapid identification of three types of triter- penoid saponins with different parent nucleuses in Ilex cornuta. Methods： HPLC - ESI - MS method was used to investigate the ESI - MS （ ＋ ） characteristics of those triterpenoid saponins, which were adopted to indentify three types of triterpenoid saponins in llex cornuta. Results： Three types of triterpenoid saponins have different ESI - MS （ ＋ ） characteristics, by which 29 triterpenoid saponins were rapidly identified from the extract of Ilex cornuta. A- mong them, 10 compounds were accurately identified by comparison with reference substances. Conclusions：HPLC - ESI - MS has been provided not only as a new thread and method for rapidly identification of triterpenoid saponins from complex system, but also an effective method for target separation of constituents in natural drugs.

关 键 词：三萜皂苷 枸骨叶(苦丁茶) 液质联用 ESI-MS裂解规律 化学筛选 结构特征 物质基础 在线快速鉴定 

分 类 号：R917[医药卫生—药物分析学]