近红外光谱法快速测定虎杖中虎杖苷、白藜芦醇、大黄素的含量  

Rapid determination of polydatin,resveratrol and emodin in Rhizoma Polygoni Cuspidati by NIRS

作　　者：吴利敏[1] 杨琼[2] 周尚[2] 蒙杰丹[1] 朱乾华[2] 张敏[1] 杨季冬[1,2,3] 

机构地区：[1]西南大学化学化工学院,北碚400715 [2]长江师范学院化学化工学院,涪陵408100 [3]重庆三峡学院化学与环境工程学院,万州404100

出　　处：《药物分析杂志》2012年第9期1583-1588,共6页Chinese Journal of Pharmaceutical Analysis

基　　金：重庆市科委攻关项目(CSTC;2010AC5170);重庆市教委科技项目(KJ111303);重庆市涪陵区科委项目(FLKJ;2011ABA2028)

摘　　要：目的:建立一种同时测定虎杖提取物中虎杖苷、白藜芦醇和大黄素含量的新方法。方法:利用高效液相色谱紫外检测法测定虎杖中活性成分的化学值,然后采用傅里叶变换近红外光谱技术并结合偏最小二乘法(PLS)建立、优化模型,最后采用校正模型的决定系数(R2)、内部交叉验证均方差(RMSECV)和外部预测误差均方根(RMSEP),对校正模型进行评价。结果:虎杖苷、白藜芦醇和大黄素定量校正模型的R2分别为0.9589,0.9604,0.9128;RMSECV分别为0.0881,0.0172,0.130;RMSEP分别为0.0968,0.0153,0.111。结论:结果证明近红外光谱法用于虎杖中活性成分的定量分析,准确度较高,能满足现实中对虎杖多组分同时测定的精度要求。Objective ：To develop a new method for synchronous determination of polydatin, resveratrol and emodin in Rhizoma Polygoni Cuspidati. Methods ： The chemical values of the active components in Rhizoma Polygoni Cus- pidati were determined by the reversed - phase high - performance liquid chromatography （ RP - HPLC ） with UV detection. The quantitative calibration models were established by Fourier transformation near - infrared spectrosco- py （NIR） combined with partial least square （PLS） regression, optimized and evaluated by coefficient of determi- nation（R2）, the root mean square of cross- validation （RMSECV） and the root mean square of prediction （RM- SEP）. Results： The quantitative calibration models of polydatin, resveratrol, ernodin were successively established with R2 =0. 9589, 0. 9604 and 0. 9128, RMSECV =0. 0881, 0. 0172 and 0. 130 and RMSEP =0. 0968, 0. 0153 and 0. 111, respectively. Conclusions ：The results showed that NIR could be used in the quantitative analysis of the active components in Rhizoma Polygoni Cuspidati with great accuracy and achieve the precision of synchronous determination of muhicomponents in Rhizoma Polygoni Cuspidati.

关 键 词：近红外光谱法 偏最小二乘法 无损测定 高效液相色谱 虎杖 蒽醌类化合物 二苯乙烯类化合物 虎杖苷 白藜芦醇 大黄素 中药多成分分析 

分 类 号：R917[医药卫生—药物分析学]