高效液相色谱同时测定多维元素片中的维生素B_1、维生素B_6、维生素C、烟酰胺和泛酸  

Simultaneous determination of vitamin B_1,vitamin B_6, vitamin C,niacinamide and pantothenic acid in vitamins with minerals tablets by HPLC

作　　者：金鹏飞[1] 夏路风[1] 李铮[1] 胡欣[1] 姜文清[1] 邹定[1] 

机构地区：[1]卫生部北京医院药学部,北京100730

出　　处：《药物分析杂志》2012年第9期1606-1610,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：中央保健科研课题专项资金(B2009B032)

摘　　要：目的:应用高效液相色谱建立同时测定多维元素片中维生素B1、维生素B6、维生素C、烟酰胺和泛酸含量的方法。方法:以0.05%(V/V)磷酸溶液和0.3%(W/V)硫代硫酸钠溶液为提取液,采用Alltima C18色谱柱(250 mm×4.6 mm,5μm),以50 mmol.L-1磷酸二氢铵溶液(磷酸调至pH 3.0)-乙腈(95∶5)为流动相,流速0.5 mL.min-1,维生素B1、维生素B6、维生素C、烟酰胺的检测波长为275 nm,泛酸的检测波长为210 nm。结果:在本方法中,5个成分的线性关系良好,线性相关系数(r)均大于0.9999;专属性强,5个成分和药品中其他成分以及强制降解产物之间均有良好的分离度;精密度良好,RSD小于2.0%;回收率良好,3个加样浓度的回收率在99.45%和100.5%之间;稳定性满足实验要求,混合对照品溶液中维生素C在常温下12 h内保持稳定,供试品溶液中维生素C在常温下2 h内保持稳定;方法学比较显示:本方法和美国药典方法测定结果一致。结论:该方法准确、简单、快速,可作为多维元素片中维生素B1、维生素B6、维生素C、烟酰胺和泛酸同时测定的方法。Objective：To establish an HPLC method for the simultaneous determination of vitamin B1, vitamin B6, vitamin C, niacinamide and pantothenic acid in vitamins with minerals tablets. Methods： Samples were extracted with a solution containing O. 05% （WV） phosphoric acid and 0. 3% （W/V） sodium thiosulfate. An Alltima C18 col- umn（250 mm×4.6 ram, 5 μm） was used for the separation with 50 mmol· L-1 ammonium dihydrogen phosphate （adjusted pH to 3.0 with H3 PO4 ） and acetonitrile （95：5） as the mobile phase at the flow rate of 0. 5 mL· min- 1 The detection wavelength was 275 nm for vitamin B1, vitamin B6, vitamin C, niacinamide, and 210 nm for panto- thenic acid. Results： The method showed good linearity for vitamin B1, vitamin B6, vitamin C, niaeinamide and pantotbenic acid with all correlation coefficients （r） greater than 0. 9999; the specificity study showed satisfactory resolutions between vitamin B1, B6, C, niacinamide, pantothenic acid, other ingredients and forced degradation products ; the precisions were satisfactory with all RSDs less than 2.0% ; the spike recoveries of three different con- centrations ranged from 99.45% to 100. 5% for all compounds; the stability at ambient temperature met the experi- mental requirements with a 12 h stabilization for vitamin C in combined standard solutions and 2 h stabilization in analytical solutions. Method comparison indicated coincident results between this method and the USP method. Conclusions ： This method is accurate, fast and simple, which can he used for the simultaneous determination of vi- tamin B1, vitamin B6 ,vitamin C, niacinamide and pantothenic acid in vitamins with minerals tablets.

关 键 词：高效液相色谱 多维元素片 善存 维他命B1、B6、C 硫胺 吡多辛 抗坏血酸 烟酰胺 泛酸 维生素微量元素复方制剂 

分 类 号：R917[医药卫生—药物分析学]