HPLC法同时测定莱阳梨清膏中3个咖啡酰奎宁酸类成分的含量  

HPLC method for simultaneous determination of three caffeoylquinic acids in Laiyang pear clear paste

作　　者：桂怡 徐丽华[2] 林永强[2] 

机构地区：[1]北京北方医院,北京100089 [2]山东省食品药品检验所,济南250101

出　　处：《药物分析杂志》2012年第9期1648-1650,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立同时测定莱阳梨清膏中3个咖啡酰奎宁酸类成分3-O-咖啡酰奎宁酸、绿原酸(5-O-咖啡酰奎宁酸)、4-O-咖啡酰奎宁酸的HPLC方法。方法:采用Thermo BDS(4.6 mm×250 mm,5μm)色谱柱,以乙腈(A)-0.4%磷酸溶液(B)为流动相,梯度洗脱(0～35 min,5%A→10%A;35～40 min,10%A),流速1.0 mL.min-1,检测波长为327 nm。结果:3-O-咖啡酰奎宁酸、绿原酸(5-O-咖啡酰奎宁酸)、4-O-咖啡酰奎宁酸进样量分别在0.023～0.230,0.022～0.224,0.023～0.226μg范围内与峰面积呈良好的线性关系;平均回收率(n=6)分别为97.2%,97.0%,99.5%。结论:本法操作简便,结果准确,灵敏度高,可用于莱阳梨清膏中多指标成分的质量控制。Objective：To develop an HPLC method for simultaneously determination of three caffeoylquinic acids （3 - O - caffeoylquinie acid, chlorogenic acid, 4 - O - caffeoylquinic acid） in Laiyang pear clear paste. Methods： Thermo BDS（4.6 mm ×250 mm,5μm）column was adopted. The mobile phase consisting of aeetonitrile（A） - 0. 4% phosphoric acid solution（B） with gradient elution （0 - 35 rain,5% A→10% A ;35 - 40 rain, 10% A） was ap- plied at a flow rate of 1.0 mL · min-1. The detection wavelength was 327 nm. Results：The calibration curves were linear in the ranges of 0. 023 -0. 230,0. 022 -0. 224,0. 023 -0. 226μg for 3 - 0 -caffeoylquinic acid, ehlorogen- ic acid（5 - 0 - eaffeoylquinie acid）, 4 - 0 - eaffeoylquinic acid; The average recoveries （ n = 6 ） were 97.2%, 97.0% and 99.5% ,respectively. Conclusion ：The method is simple, accurate, and sensitive ,which can be used for quality control of multi - component in Laiyang pear clear paste.

关 键 词：高效液相色谱法 白梨 莱阳梨清膏 绿原酸 咖啡酰奎宁酸 异构体 中成药多指标成分检测 

分 类 号：R917[医药卫生—药物分析学]