晚期糖基化终末产物对人骨髓间充质干细胞体外增殖的影响  

Effect of advanced glycosylation end products(AGEs) on proliferation of human bone marrow mesenchymal stem cells(MSCs) in vitro

作　　者：陆轶群[1] 鲁燕[1] 李慧娟[1] 成兴波[1] 

机构地区：[1]苏州大学附属第一医院内分泌科,江苏苏州215006

出　　处：《苏州大学学报（医学版）》2012年第4期514-517,523,共5页Suzhou University Journal of Medical Science

摘　　要：目的研究晚期糖基化终末产物(AGEs)对体外分离培养的人骨髓间质干细胞(MSCs)增殖的影响。方法采用CCK-8比色法检测在不同时间和浓度AGEs干预下所培养的MSCs的吸光度和增殖活力,同时测定细胞内的丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性。结果 AGEs加入细胞培养后,可显著抑制MSCs的增殖(均P<0.01),并呈剂量和时间依赖效应关系。随着AGEs-BSA作用浓度的增加,细胞内丙二醛含量明显增加,而细胞匀浆中超氧化物歧化酶的活性却受到了抑制(均P<0.01),具有剂量依赖效应。结论 AGEs通过增强MSCs内的氧化应激,破坏MSCs内环境稳定性,从而抑制MSCs的增殖。研究晚期糖基化终末产物(AGEs)对体外分离培养的人骨髓间质干细胞(MSCs)增殖的影响。Objective To explore the effect of advanced glycosylation end products （AGEs） on proliferation of human bone marrow mesenehymal stem cells （MSCs） in vitro and relevent mechanism. Methods Absorbance was assayed using CCK-8 colorimetry to analysis the proliferation activity of MSCs under the intervention of AGEs, the content of MDA and the activity of SOD were measured. Results The proliferation activity of MSCs was significantly inhibited when AGEs were added to culture medium, and the effects were dose-depedent and time-dependent. Compared with control groups, co-culturing with AGEs-BSA increased the content of MDA in cells, and simultaneously the activiey of SOD was decreased, both in a dose dependent mariner. Conclusion AGEs breaks the homeostasis of cell by increasing the oxidative stress, and accordingly, inhibit the proliferation of MSCs in vitro significantly.

关 键 词：糖基化终末产物 骨髓间充质干细胞 细胞增殖 氧化应激 

分 类 号：R392.6[医药卫生—免疫学]