离子色谱法测定复方卡托普利片中卡托普利和氢氯噻嗪的含量  

Determination of Contents of Captopril and Hydrochlorothiazide in Compound Captopril Tablets by Ion Chromatography

作　　者：吕伟德[1] 周丹[2] 朱岩[2,3] 

机构地区：[1]杭州职业技术学院,杭州310018 [2]桂林理工大学化学与生物工程学院,广西桂林541004 [3]浙江大学化学系,杭州310028

出　　处：《中国无机分析化学》2012年第3期73-75,共3页Chinese Journal of Inorganic Analytical Chemistry

基　　金：浙江省自然科学基金一般项目(Y2110945);浙江省科技项目分析测试项目(2011C37065)

摘　　要：建立离子色谱法测定复方卡托普利片中卡托普利和氢氯噻嗪含量的方法。在色谱柱为IonPacSCS1(4mm×250mm),保护柱为IonPacSCG1(4mm×50mm),淋洗液为甲烷磺酸(4mmol/L)+乙腈(26%),流速1.0mL/min,柱温35℃,进样量7μL,紫外检测波长215nm的条件下,测定结果表明,卡托普利和氢氯噻嗪在0.5～25μg/mL范围内线性关系良好,相关系数r分别为0.9997、0.9999,回收率分别为98.11%、94.24%。方法操作简单,灵敏度高、准确、重现性好,可用于复方卡托普利片的质量控制。An analytical method for quantitative determination of captopril and hydrochlorothiazide in compound captopril tablets by ion chromatography（IC） was established. The method was performed on IonPac SCS1（4 mm×250 mm） chromatographic column and IonPac SCG1（4 mm×50 mm） guard column. The chromatographic conditions were： the mixture of 4 mmol/L methanesulfonic acid solution and 26% acetonitrile was used as the mobile phase; the flow rate was 1 mL/min, the column temperature was 35 ℃; the injection volume was 7 μL, and the detection wavelength was 215 nm. The results showed that the calibration curves of captopril and hydrochlorothiazide had good linearity in the range of 0.5-25 μg/m with the correlation coefficient of 0.9997 and 0.9999, respectively. The average recovery for captopril and hydrochlorothiazide was 98.11 % and 94.24 %, respectively. The limit of detection for captopril and hydrochlorothiazide was 0.012 and 0.002 μg/mL, respectively. The method was proved to be simple, sensitive, accurate, and reliable for evaluating the quality of compound captopril tablets.

关 键 词：离子色谱法 复方卡托普利片 卡托普利 氢氯噻嗪 

分 类 号：O657.75[理学—分析化学] TH833[理学—化学]