靛玉红大鼠体内吸收药代动力学研究  

Pharmacokinetic Study of Indirubin Absorption in Vivo in Rats

作　　者：魏宇峰 

机构地区：[1]浙江省舟山市妇幼保健医院药剂科,浙江舟山316000

出　　处：《中国药业》2012年第17期16-17,共2页China Pharmaceuticals

摘　　要：目的建立测定大鼠血浆中靛玉红质量浓度的高效液相色谱法,考察靛玉红在大鼠体内的吸收动力学特征。方法大鼠单剂量口服25 mg靛玉红,采用高效液相色谱法测定血浆中靛玉红的质量浓度。结果靛玉红在大鼠体内分布符合二室模型,主要药物代谢动力学参数分别为峰浓度(Cmax)为(48.63±5.68)ng/mL,达峰时间(Tmax)为(18.76±3.55)h,分布相半衰期(t1/2α)为(38.96±9.51)h,消除相半衰期(t1/2β)为(223.81±154.69)h,0～t药时曲线下面积(AUC0-t)为(2.87±1.33)μg.h/L。结论所建立的高效液相色谱法灵敏、快速,适用于体内靛玉红的质量浓度测定及药物代谢动力学研究。Objective To establish an HPLC method for detecting the plasma concentration of indirubin in rats and to investigate the pharmacokinetic characteristics of indirubin absorption in vivo. Methods Rats orally took indirubin with single dose of 25 mg. The plasma level of indirubin was determined by HPLC. Results The distribution in vivo of indirubin in rats fit the two-compartment model. The main pharmacokinetic parameters were as follows, Cmax = （48.63 ±5.68） ng/mL; tmax = （18.76 ±3.55） h; t1/2± = （38.96 ± 9.51）h; t1/2a = （223.81 ± 154. 69）h; AUC（0-t） = （2. 87 ± 1.33）μg/（h · L）. Conclusion This established HPLC method is sensitive, rapid and suitable to the concentration detection of indirubin in vivo and the pharmacokinetic study of indirubin.

关 键 词：靛玉红 高效液相色谱法 药物代谢动力学 

分 类 号：R285.5[医药卫生—中药学] R282.71[医药卫生—中医学]