高效液相色谱法测定咳露口服液中黄芩苷含量  

Determination of Baicalin in Kelu Oral Liquid by HPLC

作　　者：苏英英 杨东花[2] 黄壮壮[2] 刘峰 

机构地区：[1]山海丹医院,陕西西安710004 [2]陕西中医学院,陕西咸阳712046 [3]陕西步长制药有限公司,陕西咸阳712000

出　　处：《中国药业》2012年第17期26-27,共2页China Pharmaceuticals

摘　　要：目的建立测定咳露口服液中黄芩苷含量的高效液相色谱法。方法色谱柱为Hibar C18柱(250 mm×4.6 mm,5μm),乙腈-0.1%磷酸溶液(24∶76)为流动相,流速为1.0 mL/min,检测波长为280 nm。结果黄芩苷进样量在0.170 6～1.364 8μg范围内与峰面积线性关系良好,线性回归方程为Y=3 406 924 X+138 099(r=0.999 6)。平均回收率为97.31%,RSD为1.024%(n=6)。结论该方法操作简单、准确、重现性好,可用于提高咳露口服液的质量控制标准。Objective To establish an HPLC method for the determination of baicalin in Kelu Oral Liquid. Methods The HPLC was performed on the Hihar C18 calumn（250mm×4.6mm, 5 μm） with acetonitrile-0. 1% phosphoric acid solution（24：76） as the mobile phase at the flow rate of 1.0 mL/min and the detection wavelength of 280 nm. Results The linear relation of baicalin during the range of 0. 170 6- 1. 364 8 Ixg with the peak area was good and the linear regression equation was Y= 3 406 924X ＋ 138 099（ r = 0. 999 6）. The average recovery rate was 97.31%, RSD was 1. 024% （n =6）. Conclusion This method is simple, accurate, better repro- ducible and can be used to improve the quality standard of Kelu Oral Liauid.

关 键 词：咳露口服液 黄芩苷 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学] R286.0[医药卫生—中医学]