毛冬青皂苷B_3的抗血栓作用研究  

Antithrombotic activity of ilexsaponin B_3 from roots of Ilex pubescens

作　　者：熊天琴[1] 陈元元[1] 李红侠[1] 林朝展[1,2] 祝晨蔯[1,2] 赵钟祥[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006 [2]广州中医药大学广中尖峰天然产物共建实验室,广东广州510006

出　　处：《中草药》2012年第9期1785-1788,共4页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(30901954);广东省自然科学基金资助项目(9451040701003203);高等学校博士学科点专项科研基金(20094425110008);广州中医药大学创新基金(09CX015);广州市珠江科技新星专项(2011J2200047)

摘　　要：目的从毛冬青根中分离、鉴定毛冬青皂苷B3,研究其抗血栓作用。方法色谱法、重结晶法分离、精制毛冬青皂苷B3,薄层色谱对照及波谱分析法对毛冬青皂苷B3进行结构鉴定。采用体内血栓形成试验、体外血凝块溶解试验及二磷酸腺苷(ADP)诱导血小板聚集试验,观察毛冬青皂苷B3的抗血栓作用。结果体内抗血栓试验显示,毛冬青皂苷B3对胶原蛋白-肾上腺素诱发的血栓形成所致小鼠的偏瘫和死亡及FeCl3诱导的大鼠腹主动脉血栓形成均有明显的抑制作用;在体外实验中,对家兔血凝块无明显的溶解作用,对ADP诱导的离体家兔血小板聚集也无明显的抑制作用。结论毛冬青皂苷B3可能为毛冬青抗血栓作用的物质基础之一,其体内抗血栓形成的作用可能与其促进血凝块溶解和抗ADP诱导的血小板聚集无关。Objective To separate and prepare ilexsaponin B3 （IB3） from the roots of llexpubescens, and to study its antithrombotic activity. Methods Chromatography and recrystallizafion were used in the separation and purification of IB3, and the structure was identified by PTLC and ^1H-NMR. Antithrombotic activity ofIB3 was observed by in vivo thrombosis test, solubility test of blood clot in vitro, and platelet aggregation in rabbits induced by adenosine diphosphate （ADP）. Results IB3 had significant inhibition on thrombosis-caused hemiplegia and death induced by collagen-epinephrine in mice, and on abdominal arterial thrombotic model induced by FeCl3 in rats. IB3 neither had dissolution solubility experiment of blood clot in rabbits nor had inhibition on platelet aggregation in rabbits induced by ADP. Conclusion IB3 may be one of the ilexolic material bases for the antithrombotic activity, but it may have no correlation with increasing thrombolysis and platelet aggregation induced by ADP.

关 键 词：毛冬青 毛冬青皂苷B3 抗血栓 血栓形成 血小板聚集 

分 类 号：R284.10[医药卫生—中药学] R285.5[医药卫生—中医学]