吴茱萸Mn/Fe-SOD基因全长cDNA克隆及序列分析  

Full-length cDNA cloning and sequence analysis of Mn/Fe superoxide dismutase gene in Evodia rutaecarpa

作　　者：吴波[1] 罗光明[1] 潘超美[2] 张寿文[1] 

机构地区：[1]江西中医学院江西省中药种质资源工程技术研究中心,江西南昌330004 [2]广州中医药大学中药学院,广东广州510006

出　　处：《中草药》2012年第9期1814-1817,共4页Chinese Traditional and Herbal Drugs

基　　金：国家"十二五"科技支撑计划项目"龙脑樟等6种大宗药材规范化种植基地及其SOP优化升级研究"(2011BAI04B04)

摘　　要：目的克隆吴茱萸Mn/Fe-SOD基因的全长cDNA序列。方法根据已获得的吴茱萸Mn/Fe-SOD基因核心片段序列设计4条特异性引物,采用RACE和巢式PCR方法克隆获得吴茱萸Mn/Fe-SOD基因全长cDNA。结果吴茱萸Mn/Fe-SOD基因全长cDNA序列共1 048 bp,包含一个687 bp的开放阅读框(open reading frame,ORF),共编码228个氨基酸。结论首次从吴茱萸中克隆得到了Mn/Fe-SOD基因的全长cDNA序列,为研究该基因在吴茱萸体内超量表达以提高植物抗逆性研究奠定基础。Objective To clone the full-length cDNA sequence of Mn/Fe superoxide dismutase （Mn/Fe-SOD） gene of Evodia rutaecarpa. Methods Four gene specific primers were designed according to the previously obtained core sequence of Mn/Fe-SOD gene fragment in E. rutaecarpa. RACE and nested PCR technologies were used to clone the full-length cDNA of Mn/Fe-SOD gene in E. rutaecarpa. Results The full-length cDNA sequence of Mn/Fe-SOD gene composed of 1 048 bp was obtained including an open reading frame （ORF） with 687 bp corresponding to 228 amino acids. Conclusion The full-length cDNA sequence of Mn/Fe-SOD gone in E. rutaecarpa is cloned and reported for the first time, which lays a foundation for researching the gene excess expression in order to increase stress resistance ofE. rutaecarpa.

关 键 词：吴茱萸 MN Fe—SOD基因克隆 RACE 开放阅读框 序列分析 

分 类 号：R282.12[医药卫生—中药学]