山西浑源仿野生栽培蒙古黄芪的质量研究  

Quality analysis of semi-cultivated Astragalus membranaceus var. mongholicus from Hunyuan County of Shanxi Province

作　　者：胡明勋[1,2] 郭宝林[1] 周然[3] 黄文华[1] 曹秀娟 侯美利 陈安家[2] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193 [2]山西医科大学,山西太原030001 [3]山西中医学院,山西太原030001 [4]山西省浑源县科技局,山西浑源037400

出　　处：《中草药》2012年第9期1829-1834,共6页Chinese Traditional and Herbal Drugs

基　　金：山西省科技攻关项目(2561);国家科技支撑计划项目(2011BAI07B01)

摘　　要：目的通过对山西浑源县仿野生栽培和野生蒙古黄芪中皂苷类和黄酮类成分的比较,评价仿野生栽培蒙古黄芪的质量。方法采集12批野生和19批仿野生栽培蒙古黄芪样品,采用HPLC-DAD法测定毛蕊异黄酮苷、芒柄花苷、毛蕊异黄酮、芒柄花素4种黄酮成分,UPLC-ELSD法测定黄芪皂苷I、II、III、IV 4种皂苷成分。结果 5～6年生的仿野生栽培的蒙古黄芪中的高质量分数成分毛蕊异黄酮苷、芒柄花苷、黄芪皂苷I、II、III的量高于野生黄芪,相应的黄酮总量、皂苷总量高于野生黄芪,而2种低质量分数成分芒柄花素、黄芪皂苷IV的量低于野生黄芪;通过比较仿野生栽培1～6年生黄芪中8种成分的量发现,黄酮类成分随生长年限的增长不断升高,而皂苷类成分则逐渐降低。结论仿野生栽培的方法可有效地解决优质野生浑源蒙古黄芪资源不足的问题。Objective To evaluate the quality ofsemi-cultivatedAstragalus membranaceus var. mongholicus through comparing the contents of flavonoids and saponins between the semi-cultivated and wild A. membranaceus var. mongholicus in Hunyuan County, Shanxi Province. Methods Twelve wild samples and 19 semi-cultivated samples of A. membranaceus var. mongholicus were collected. The contents ofcalycosin glucoside, ononin, calycosin, and formononetin were analyzed by HPLC-DAD, and astragalosides Ⅰ, Ⅱ, Ⅲ, and Ⅳ by UPLC-ELSD. Results The average contents ofcalycosin glucoside, ononin, astragalosides Ⅰ, Ⅱ, and Ⅲ in five- or six-year semi-cultivated plants were higher than those in wild plants, so were the total flavonoids and total saponins. However, other two formononetin, and astragaloside Ⅳ were less than those in the wild; Based on the comparison of eight ingredients in the one- to six-year semi-cultivated plants, the contents of flavonoids continuously increased with the growth years increasing, while those of saponins were lower along with the growth period. Conclusion The semi-cultivated method to solve the resources shortage of high quality wild A. membranaceus var. mongholicus in Hunyuan is effective and reliable.

关 键 词：蒙古黄芪 野生 仿野生 黄酮 皂苷 

分 类 号：R286.02[医药卫生—中药学]