感染斯氏狸殖吸虫大鼠IL-4、IL-6、TSLP、TGF-β1水平检测  

The Level of IL-4,IL-6,TSLP and TGF-β1 in Rat Infected by Paragonimus Skrjabini

作　　者：吴玛莉 李安梅 唐莉娜 徐莉娜 卢丽丹 潘中明 郭志来[3] 

机构地区：[1]贵州省疾病预控控制中心,贵阳550004 [2]杭州联科生物研发中心 [3]贵州师范大学

出　　处：《职业卫生与病伤》2012年第4期230-232,共3页Occupational Health and Damage

基　　金：贵州省优秀科技教育人才省长资金项目(项目合同编号:黔省专合字2010-91号)

摘　　要：目的研究斯氏狸殖吸虫感染引起的SD大鼠Th2免疫反应产生的细胞因子IL-4、IL-6、TSLP、TGF-β1水平,探寻寄生虫感染所致大鼠细胞免疫反应情况。方法用斯氏狸殖吸虫囊蚴感染SD大鼠5只,于感染后16周对其血清进行IL-4、IL-6、TSLP、TGF-β1的ELISA检测。结果 IL-4、TSLP、TGF-β1无显著变化,分别为0.34 pg/ml、8.69 pg/ml、2 06681.86 pg/ml(P>0.05),IL-6显著升高到40.45 pg/ml,具有统计学意义(P<0.05)。结论感染SD大鼠Th2免疫反应活化,IL-6表达显著上调。Objective To detect the cytokine levels of IL - 4, IL - 6, TSLP and TGF -β1 of Th2 immune re- sponse in SD rats infected by Paragonimus skrjabini, and to understand cell-mediated immune response of para- sites infecting. Methods Five SD rats were infected by metacereariae of Paragonimus skrjabini. After 16 weeks infection, the IL-4, IL-6, TSLP and TGF-β1 in rat serum were tested by ELISA. Results The IL-4, IL - 6 and TSLP had no significant change （ P 〉 0.05 ）, with concentrations of 0. 34 pg/ml, 8.69 pg/ml and 206 681.86 pg/ml, respectively. IL- 6 increased to 40. 45 pg/ml and showed statistical significance （P 〈 0. 05 ）. Conclusions Th2 immune response of infected SD rats was activated, and the expression of IL - 6 signifi- candy rose.

关 键 词：斯氏狸殖吸虫 IL-4 IL-6 TSLP TGF-Β1 

分 类 号：R392.11[医药卫生—免疫学]