大豆膨胀素GmEXPA4基因的克隆及表达分析  

Cloning and expression of expansin GmEXPA4 gene in soybean

作　　者：闻静[1] 李凤兰[1] 赵刚 孙丽[1] 孙莉莉[1] 胡宝忠[1] 

机构地区：[1]东北农业大学生命科学学院,黑龙江哈尔滨150030 [2]黑龙江省丰合种业,黑龙江哈尔滨150030

出　　处：《中国油料作物学报》2012年第4期343-347,共5页Chinese Journal of Oil Crop Sciences

基　　金：黑龙江省自然科学基金(230340);黑龙江省教育厅科学技术研究项目(12511061)

摘　　要：根据植物膨胀素基因保守区设计简并引物,以大豆矮化突变体HK808幼芽的cDNA为模板,克隆到530bp的膨胀素基因保守区片段,并结合RACE技术克隆得到膨胀素基因全长序列,将其命名为GmEXPA4基因。生物信息学分析结果表明,该基因属于膨胀素中的α-expansin亚家族。GmEXPA4基因的实时荧光定量相对表达分析表明,该基因在东农42及其矮化突变体HK808中的各个时期都有表达,且与植物的生长量密切相关。Degenerate primers were designed according to expansin gene sequences from other plants.Conserved sequence of expansin gene in Glycine max L.was cloned using young shoot cDNA of a dwarf mutant HK808.Full-length expansin gene,designated as GmEXPA4,was cloned by RACE.Bioinformatics result showed that GmEXPA4 was a member of alpha-expansin subfamily.Fluorescence real-time quantification PCR indicated that GmEXPA4 expression was constitutively in different period in wildtype Dongnong 42 and mutant HK808.The relative expression showed close relation to the amount of soybean growth.

关 键 词：大豆 矮化突变体 膨胀素 克隆 实时荧光定量 器官表达 

分 类 号：S565.103[农业科学—作物学]