^(60)Co伽马射线辐照对苏子生长及生理特性的影响  

Effects of seed ^(60)Co gamma ray irradiation on seedling growth and physiological status of Perilla frutescens

作　　者：谭美莲[1] 汪磊[1] 严明芳[1] 王力军[1] 严兴初[1] 

机构地区：[1]中国农业科学院油料作物研究所,农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062

出　　处：《中国油料作物学报》2012年第4期396-401,共6页Chinese Journal of Oil Crop Sciences

基　　金：油料作物种质资源收集与创新利用(2006-G8(4)-18);农业部公益性行业(农业)科研专项(201003057);国家自然科学基金(31000726);中国农科院油料所所长基金(1610172011009)

摘　　要：为创新苏子(Perilla frutescens L.Britt)种质资源及为苏子辐射诱变育种提供技术参考,研究不同剂量60Co伽马射线(0、200、400、600和800Gy)照射条件下,兴仁花苏和山甲苏子种子的萌发率、田间出苗率和幼苗高度、活力指数以及MDA含量、过氧化物酶(POD)和超氧化物歧化酶(SOD)活性。结果表明,辐射对苏子种子发芽率影响不明显,但对田间出苗率、幼苗高度、活力指数及相关生理指标都存在极显著影响。在800Gy范围内,随着辐射剂量的增强,田间出苗率先缓慢下降后急剧下降,幼苗植株变矮,活力指标降低;而幼苗MDA含量、SOD活性及POD活力均随剂量的增加而不断上升。兴仁花苏比山甲苏子对辐照更为敏感,其田间出苗率急剧下降时的剂量比后者低,其幼苗的生长和生理指标(MDA含量、SOD活性及POD活性)受辐照影响均比山甲苏子明显,其理论半致死剂量和理论临界剂量也比后者低。10Gy/min剂量率下,兴仁花苏的半致死剂量和临界剂量分别为512.37Gy和570.80Gy,山甲苏子的则分别为679.74Gy和716.79Gy。In order to enhance the germplasm of Perilla frutescens and provide the radiation mutation technical reference for this species,two kinds of variety seed,Xingrenhuasu and Shanjiasuzi,were irradiated with 60Co gamma ray at the doses of 0,200,400,600 and 800Gy,and some of the physiological parameters,such as seed germination,field seedling emergence,seedling height,and plant vitality index as well as malondialdehyde（MDA） content,peroxidase（POD）,and superoxide dismutase（SOD） activities were investigated.The results showed that irradiation of the seeds significantly influenced field seedling rate,seedling height,vitality index and related antioxidant indexes,but not seed germination.Below the dose of 800Gy,with the gradually increased irradiation doses,the seedling emergence was decreased slowly then sharply,seedlings became dwarf,and the vitality index was reduced,however,the content of MDA,activities of POD and SOD were all increased.Xingrenhuasu was more sensitive to 60Co gamma ray irradiation than Shanjiasuzi,the dose that reflected on the sharp reduction of seedling emergence was lower for which than that for Shanjiasuzi,the effects on the seedling growth and three physiological indices of which were more evident than that of Shanjiasuzi,moreover its half lethal dose（LD50） and critical dose（LD40） were much lower than that of Shanjiasuzi.At the irradiation rate of 10Gy/min,the half lethal dose（LD50） and the critical dose（LD40） were 512.37Gy and 570.80Gy for Xingrenhuasu,and 679.74Gy and 716.79Gy for Shanjiasuzi,respectively.

关 键 词：苏子 辐射育种 萌发 生长 生理状况 半致死剂量 临界剂量 

分 类 号：S565.8[农业科学—作物学] S567.2