中药小茴香抗纤维化及对肝纤维化腹水大鼠补钾作用的实验研究  

Experimental study of Chinese herb foeniculum vulgare mill on liver hepatic fibrosis and potassium supplement in rats

作　　者：王磊[1,2] 张韬[1] 张建龙[1] 于洋[1] 甘子明[1] 

机构地区：[1]新疆医科大学基础医学院人体解剖教研室,乌鲁木齐830011 [2]解放军474医院普外科,乌鲁木齐830000

出　　处：《新疆医科大学学报》2012年第9期1175-1179,共5页Journal of Xinjiang Medical University

基　　金：新疆名医名方与特色药剂学实验室开放基金(XJDX0910-2010-03)

摘　　要：目的探讨肝纤维化腹水大鼠消腹水过程中小茴香的补钾作用及抗纤维化作用。方法选雄性SD大鼠100只,随机取9只设为正常对照组(A组),其余用四氯化碳(CCl4)复合因素法复制肝纤维化大鼠模型,模型复制成功后随机分为模型对照组(B组)、速尿组(C组)、速尿+氯化钾组(D组)、速尿+丹参组(E组)、速尿+小茴香低剂量组(F组)、速尿+小茴香中剂量组(G组)、速尿+小茴香高剂量组(H组),每组13只造模组(91只),用药干预6w后,镜检肝组织形态学改变;检测腹水;全自动生化分析仪检测外周血清钾及血清透明质酸酶(HAase)的含量。结果 HE结果显示:模型对照组肝脏组织炎症与脂肪变性明显,经丹参和小茴香不同剂量干预后明显改善。与正常对照组比较,模型对照组腹水和血清透明质酸酶显著增多(P<0.05),血清钾显著降低(P<0.05)。与模型对照组比较,D组、E组、F组、G组、H组腹水和血清透明质酸酶均显著减少(P<0.05),D组和F组血清钾显著升高(P<0.05)。结论中药小茴香具有良好的抗肝纤维化作用,且中药小茴香在肝硬化腹水大鼠消腹水过程中有补钾作用。Objective To observe the effects of foeniculum vulgare mill on liver fibrosis and potassium sup- plement. Methods One hundred wister rats were randomly divided into 8 groups as control group, model group, foeniculum vulgare mill group （low dose, middle dose, high dose） and therapeutic group. Liver fibrosis models were established by the combination of injecting carbon tetrachloride （CCl4） subcutaneously, feeding high lipid diet and drinking 10% alcohol. Drugs were lavaged for 6 weeks, rats livers tissue were examined with HE staining; the draining ascites was collected; the serum level of potassium and Hyaluronidase （HAase） were determined by corresponding test kits. Results Liver tissues inflammation and se- vere fatty degeneration showed in model control group, and which improved significantly after therapy by different doses of foeniculum vulgare mill; Compared with normal group, ascites level and HAase content remarkablely increased, level of potassium decreased （P 〈0.05）. Compared with model group, foenicu- lum vulgar emill group, scites level and HAase content remarkablely decreased, the level of potassium remarkablely increased （P 〈0.05）. Conclusion Foeniculum vulgate mill can inhibit the hepatic fibrosis and add the potassium lost with ascites.

关 键 词：小茴香 肝纤维化 透明质酸酶 补钾 

分 类 号：R965[医药卫生—药理学] R575[医药卫生—药学]