医院病房环境真菌监测分析  

Surveillance on Fungi in hospital environment

作　　者：吕小林[1] 章登珊[1] 曹先伟[2] 

机构地区：[1]南昌大学第一附属医院检验科,江西南昌330006 [2]南昌大学第一附属医院感染科,江西南昌330006

出　　处：《中国微生态学杂志》2012年第9期805-807,共3页Chinese Journal of Microecology

基　　金：江西省科技厅课题(JX01573)

摘　　要：目的调查医院各病区环境中真菌含量及分布特点。方法运用分离培养及DNA测序方法对医院不同病区、不同环境中真菌进行监测分析。结果医院不同病区真菌含量不同,呼吸科、血液科、小儿科空气中真菌含量较高,分别为400、225和200 CFU/m3,其中以烟曲霉菌(325 CFU/m3)、黄曲霉菌(275 CFU/m3)、枝孢霉菌(125 CFU/m3)、根霉(125 CFU/m3)为主;老年病科、肿瘤科和血液科空调出气口真菌含量较高,分别为0.559、0.500和0.323 CFU/cm2,以链格孢霉(0.441 CFU/cm2)、根霉(0.412 CFU/cm2)、烟曲霉菌(0.294 CFU/cm2)为主;不同环境中真菌含量不同,其中以空气和空调出气口真菌含量最高,分别为130 CFU/m3、0.173 CFU/cm2。结论真菌广泛存在于医院环境中,且不同病区、不同环境真菌污染程度不同,因而我们必须制订健全的消毒管理制度,预防医院真菌感染。Objective To survey the fungal density and distribution characteristics in wards of the hospital.MethodsFractional cultivation and DNA sequencing method were used to analyze fungi in hospital environment.Results In the air of respiratory,haematology and paediatrics wards,the fungal densities were higher than that of other wards,which were 400 CFU/m^3,225 CFU/m^3 and 200 CFU/m^3 respectively,including mainly Aspergillus fumigatus（325 CFU/m^3）,Aspergilus flavus（275 CFU/m^3）,Branch spore mold（125 CFU/m^3） and Rhizopus（125 CFU/m^3）.At the air vent of air conditioning in geriatrics,oncology and haematology departments,fungal densities were higher,at 0.559 CFU/cm^2,0.5 CFU/cm^2 and 0.323 CFU/cm^2 respectively,which were mainly composed of Alternaria（0.441 CFU/cm^2）,Rhizopus（0.412 CFU/cm^2） and Aspergillus fumigatus（0.294 CFU/cm^2）.The samples collected from air and air conditioning vent showed the highest concentrations of fungi,at 130 CFU/m^3 and 0.173 CFU/cm^2 respectively.Conclusion Fungi exist extensively in the hospital,and its contamination varies in different environments.The cleansing and disinfection systems must be further improved to prevent nosocomial fungus infection.

关 键 词：病房 真菌 环境监测 

分 类 号：R379[医药卫生—病原生物学]