29株耐亚胺培南和/或美罗培南鲍曼不动杆菌产碳青霉烯酶基因型检测  

The genetype of carbapenemase produced by imipenem-and/or meropenem-resistant Acinetobacter baumannii isolates

作　　者：魏艳艳[1] 熊自忠[2] 邹桂舟[1] 

机构地区：[1]安徽医科大学第二附属医院肝病科,安徽合肥230000 [2]安徽医科大学第一附属医院感染科,安徽合肥230022

出　　处：《中国微生态学杂志》2012年第9期808-809,818,共3页Chinese Journal of Microecology

基　　金：安徽省自然科学基金(050430804);安徽省优秀青年科技基金(08040106815)

摘　　要：目的了解临床分离耐亚胺培南和/或耐美罗培南鲍曼不动杆菌中产碳青霉烯酶的基因型别。方法采用聚合酶链反应扩增IMP、VIM、OXA型碳青霉烯酶基因并测序。结果 29株对碳青霉烯类耐药的鲍曼不动杆菌中,以产OXA-24型和IMP型酶菌株最多,二者均占51.7%(15/29)。产OXA-24+IMP型5株、OXA-24+OXA-51+IMP型4株、VIM型4株、OXA-24+OXA-58+IMP型、OXA-23+OXA-24+IMP型各2株,OXA-23+IMP型、OXA-51+OXA-24型、OXA-24型、IMP型各1株,8株细菌PCR检测结果为阴性。结论耐亚胺培南和/或美罗培南鲍曼不动杆菌主要产OXA-24型和IMP型碳青霉烯酶,部分菌株可同时产2种或以上碳青霉烯酶。Objective To investigate the genetype of carbapenemase produced by imipenem-resistant and/or meropenem-resistant Acinetobacter baumannii strains clinical isolated.Methods A total of 29 strains of imipenem-resistant and/or meropenem-resistant Acinetobacter baumannii were screened by PCR with primer pairs designed to detect genes encoding OXA-23,24,51,58 and VIM,IMP enzymes,respectively.Results In the 29 carbapenemase producers,OXA-24 and IMP were the most common,each accounting for 51.7%（15/29）.The positive strains of OXA-24＋IMP,OXA-24＋OXA-51＋IMP,VIM,OXA-24＋OXA-58＋IMP,OXA-23＋OXA-24＋IMP,OXA-23＋IMP,OXA-51＋OXA-24,OXA-24 and IMP were 5,4,4,2,2,1,1,1 and 1 strain,respectively.Conclusion OXA-24 and IMP are the main genotypes of carbapenemase in imipenem-resistant and/or meropenem-resistant Acinetobacter baumannii.

关 键 词：鲍曼不动杆菌 碳青霉烯酶 基因型 

分 类 号：R378[医药卫生—病原生物学]