胰岛素受体底物1在前成骨细胞分化过程中的表达  

The Change of IRS-1 Expression during the Differentiation of MC3T3-E1 Cells

作　　者：何玉玲[1] 周后德[2] 隋国良[2] 刘敏[2] 黄秋霞[2] 廖二元[2] 

机构地区：[1]广西医科大学第一附属医院代谢糖尿病中心,南宁530021 [2]中南大学湘雅二医院代谢内分泌研究所,长沙410011

出　　处：《中国医科大学学报》2012年第9期769-772,共4页Journal of China Medical University

基　　金：国家自然科学基金资助项目(30400218)

摘　　要：目的研究胰岛素受体底物1(IRS-1)在小鼠前成骨样MC3T3-E1细胞分化过程中的表达。方法在小鼠前成骨样MC3T3-E1细胞分化成熟过程中,分别在不同时点收集细胞及上清液。骨钙素含量用放射免疫法测定;碱性磷酸酶活性用α-磷酸奈酚法测定;Ⅰ型胶原、骨涎蛋白、骨桥素和IRS-1的mRNA表达应用半定量RT-PCR检测;免疫印迹法测定IRS-1蛋白水平。结果在小鼠前成骨样MC3T3-E1细胞分化过程中,IRS-1的表达量逐渐增加,而且与Ⅰ型胶原、骨钙素、骨涎素和骨桥素呈正相关。结论 IRS-1参与MC3T3-E1细胞的增殖、成熟与矿化的全过程。Objective To investigate the expression profile of insulin receptor substrate 1（IRS-1） during the differentiation of preosteoblastic cells.Methods Pnitrophenyl phosphate assay was used to evaluate alkaline phosphatase activity,RIA was used to evaluate osteocalcin.Semi-quantitive RT-PCR was used to examine the mRNA levels of bone sialoprotein（BSP）,osteopontin（OPN）,collagen type Ⅰ（Col Ⅰ）and IRS-1 in MC3T3-E1 cells.Western blot was adopted to evaluate the level of IRS-1 protein.Results During differentiation of MC3T3-E1 cells,IRS-1 mRNA and protein levels gradually increased.Osteocalcin,Col Ⅰ,OPN and BSP were positively related to IRS-1.Conclusion IRS-1 may be involved in the proliferation,differentiation and mineralization of murine preosteoblastic MC3T3-E1 cells.

关 键 词：MC3T3-E1细胞 胰岛素受体底物1 分化 

分 类 号：R58[医药卫生—内分泌]