基于层次-向量法分析的普通鹿蹄草品质与生态因子的相关性  

Correlation between quality of Pyrola decorata and its ecological factors based on hierarchy- vector analysis

作　　者：吕振江[1] 王冬梅[1] 李登武[1] 

机构地区：[1]西北农林科技大学林学院,陕西杨凌712100

出　　处：《植物生态学报》2012年第9期992-1003,共12页Chinese Journal of Plant Ecology

基　　金：国家林业公益性行业科研专项(20090-4004);西北农林科技大学"青年学术骨干支持计划"(Z111020902)项目资助

摘　　要：采用层次-向量法,从生态因子、有效成分含量、抗氧化活性3个层次,分析了层次间关系,研究了生态因子对普通鹿蹄草(Pyroladecorata)品质的影响,揭示影响其有效成分含量的生态主导因子。结果表明:不同地区普通鹿蹄草的4种有效成分含量及抗氧化活性差异显著。抗氧化活性(DPPHIC50值)与单宁、金丝桃苷、槲皮素含量呈负相关关系,其相关系数分别为:–0.829、–0.378和–0.749,与总黄酮含量表现为正相关(p=0.260)。单宁含量和槲皮素含量是影响抗氧化活性的重要指标,其含量增加时DPPHIC50值下降,抗氧化活性增强。年平均气温、一月份平均气温、年积温、年极高气温、无霜期、土壤全氮、速效氮、速效磷和有机质是影响有效成分含量的主要因子,其中土壤因子对有效成分含量的影响更显著。土壤因子对单宁和槲皮素含量的影响最大,对金丝桃苷含量影响最小;气候因子对4种有效成分含量影响较弱且对各成分的影响程度基本相同。研究结果表明,选择合适的生境,尤其是土壤因子可有效地提高普通鹿蹄草的品质。Plant secondary metabolites have played a significant role in drug discovery and development. Their pro- duction and accumulation are affected by environmental factors. Our objective was to determine the dominant ecological factors influencing functional components content of Pyrola decorata, the relationships among eco- logical factors, the contents of functional components and antioxidant activity. Method The HPLC method was used to determine the contents of tannin, quercetin and hyperoside. Total fla- voniods content was evaluated by NaNO2-AI（NO3）3 spectrophotometric determination. The antioxidant activity was measured by DPPH radical assay in vitro. The hierarchy-vector analysis was applied to determine relation- ships between the quality of P. decorata and ecological factors. Important findings The contents of functional components as well as the antioxidant activity in different re- gions were significantly different. Tannin, quercetin and hyperoside as the key factors affecting the antioxidant activity showed negative correlation with the antioxidant ability; their correlation coefficients were -0.829, -0.378 and -0.749, respectively, There was a positive correlation between DPPH^cs0 value and the total flavoniods con- tent （p = 0.260）. DPPHIcs0 values decreased while the functional components contents increased, which indicated the enhancement of antioxidant activity. Nine ecological factors contributed significantly to the functional com- ponents contents： annual mean air temperature, January mean air temperature, annual accumulated air tempera- ture, annual extreme high air temperature, frost-free period, soil total nitrogen, available nitrogen, available phos- phorus and organic matter. The soil factors made greater contributions to the contents of the four effective com- ponents than did climatic factors, and they also had a large impact on the content of tannin and quercetin com- pared with hyperoside. Therefore, selecting a suitable habitat, especially appropriate soil, could effectively i

关 键 词：生态因子 层次-向量分析法 普通鹿蹄草 

分 类 号：S567.239[农业科学—中草药栽培]