不同程度低氧对体外培养人胃粘膜上皮细胞株GES-1中MICA表达的影响  

EFFECT OF DIFFERENT DEGREES OF HYPOXIA ON MICA EXPRESSION IN HUMAN GES-1 CELL STRAIN

作　　者：蒋虹[1] 杨生玺[2] 张晓岩[2] 李玉琴[1] 侯成荣[2] 王明宁[2] 王文青[2] 张昱[2] 耿排力[2] 

机构地区：[1]青海大学附属医院 [2]青海大学医学院基础医学部

出　　处：《青海医学院学报》2012年第3期175-178,共4页Journal of Qinghai Medical College

基　　金：青海省科技厅应用基础研究项目(2010-Z-727)

摘　　要：目的研究不同程度低氧对体外培养人胃粘膜上皮细胞株GES-1中MICA基因表达的影响。方法建立GES-1细胞体外低氧培养模型。采用RT-PCR和免疫细胞化学技术分别检测模型MICA基因和蛋白的表达变化。结果在缺氧环境下培养0、48和72 h后MICA蛋白表达(57.67±2.30、96.14±1.75、259.59±3.51)及mRNA/β-actin比值(0.22±0.03、0.37±0.02、0.68±0.03)都随缺氧时间不同表达量存在差别(P<0.05),且表达量随缺氧时间延长而升高,直线相关分析显示二者表达呈显著正相关。结论低氧可以刺激胃上皮细胞株GES-1中MICA基因的表达量显著增加。Objective To investigate the influence of different degrees of hypoxia on Major Histocompatibility Complex Class I-related Chain A（MICA）expression in Gastric Epithelial cell Strain-1（GES-1）.Methods Hypoxia culture model in vitro was established to test the expression of MICA in GES-1 cell strain.After cultured in hypoxia circumstance for a given period of time（0hr,48hrs,72hrs）,MICA expression was tested in mRNA level and protein level by RT-PCR and immunocytochemistry analysis.Results MICA expressions increased after 0 hr,48 hrs,72 hrs in hypoxia（P〈0.05）.MICA protein expressions（57.67±2.30,96.14±1.75,259.59±3.51,respectively）increased equally with mRNA（0.22±0.03,0.37±0.02,0.68±0.03,respectively）confirmed by immunocytochemistry and RT-PCR and showed positive correlation with linear correlation analysis.Conclusion MICA gene and protein can be induced remarkably by hypoxia in GES-1 strain.

关 键 词：低氧 人胃粘膜上皮细胞 主要组织相容性复合物Ⅰ类链相关基因A 

分 类 号：R349[医药卫生—基础医学]