微量胃黏膜IL-1β mRNA水平Real-time PCR检测方法的建立  

Development Real-time PCR assay for IL-1β mRNA from micro amount gastric mucosa

作　　者：李娟[1] 嘉红云[1] 王忠英[1] 周强[1] 王方金[2] 何蕴韶[2] 吴晓蔓[1] 

机构地区：[1]广州医学院第二附属医院检验科,广东广州510260 [2]中山大学达安基因诊断中心,广东广州510080

出　　处：《中国实验诊断学》2012年第8期1430-1433,共4页Chinese Journal of Laboratory Diagnosis

摘　　要：目的建立微量IL-1βmRNA表达水平的Real-time PCR检测法。方法 20例消化道疾病患儿的胃粘膜用来做本次研究。IL-1βmRNA水平检测使用Real-time PCR荧光探针法,保守基因GAPDH做内参,采用比较循环CT法分析IL-1βmRNA的表达量。结果运用IL-1βmRNA Real-time PCR荧光探针法检测出了20例消化道疾病患儿胃粘膜IL-1βmRNA的表达量。结论成功建立了微量胃粘膜IL-1βmRNA水平Real-time PCR检测法,为IL-1β在胃肠道疾病发生发展过程中的研究提供了有力工具。Objective To develop Real-time PCR assay for IL-1β mRNA expressi on level.Methods Gastric mucosas from 20 children with gastrointestinal diseases were used in this study.IL-1β mRNA level was tested by Real-time PCR-fluore scent probe assay,conserved gene GAPDH was used as internal reference,expression of IL-1β was analyzed by ddCT method. Results IL-1β mRNA of gastric mucosa fr om 20 children with gastrointestinal diseases were detected by Real-time PCR-f luorescent probe method.Conclusion Successfully developed Real-time PCR-fluoresce nt probe assay for IL-1β mRNA from micro amount gastric mucosa and provided a powerful tool for studying IL-1β in the development of gastrointestinal diseas es.

关 键 词：胃粘膜 IL-1ΒMRNA 实时荧光定量PCR 儿童 

分 类 号：R440[医药卫生—诊断学]