小鼠菌群失调腹泻模型的建立及超微七味白术散的疗效  

The construction of mice diarrhea model due to dysbacteriosis and curative effect of ultra-micro Qiweibaizhusan

作　　者：曾奥[1] 张华玲[1] 谭周进[1] 蔡莹[1] 蔡光先[1] 周赛男[1] 

机构地区：[1]湖南中医药大学,2010级研究生湖南长沙410208

出　　处：《微生物学通报》2012年第9期1341-1348,共8页Microbiology China

基　　金：国家自然科学基金项目(No.81173214);湖南省科技厅重点项目(No.2010SK2002);湖南省研究生科研创新项目(No.CX2011B340)

摘　　要：【目的】建立菌群失调腹泻的造模方法,研究超微七味白术散对其疗效。【方法】采用抗生素联用、中药复方等不同方法进行腹泻造模,通过超微七味白术散进行治疗。【结果】中药大承气汤能快速致泻,腹泻程度最严重,久灌能出现脾虚症候,但对肠道微生物影响不大(P>0.05)。抗生素联用组中,硫酸庆大霉素和头孢拉定联用组造模效果最好,两者联用,抗菌谱达到最大互补,且混用不使药物失效。经超微七味白术散灌胃治疗后,中药组迅速治愈,脾虚症候消失。抗生素联用组小鼠肠道乳酸菌、真菌数显著高于正常组(P<0.01)。【结论】建立了一种菌群失调腹泻的造模方法,七味白术散中存在促使乳酸菌、真菌生长的益生元。[Objective] In order to construct dysbacteriotic mice model of diarrhea and understand the curative effect of ultra-micro Qiweibaizhusan.[Methods] The dysbacteriotic mice models were constructed by using traditional Chinese medicine compound and combination antibiotics to cause diarrhea,and then treated with ultra-micro powder of Qiweibaizhusan.[Results] Traditional Chinese medicine dachengqitang had quick effect on diarrhea.The symptom of insufficiency of the spleen would appeared in mice with long time traditional Chinese medicine dachengqitang treatment.But it had low effect on intestinal microbes（P〉0.05）.The combining using of gentamycin sulfate and cefradine had the best effect on diarrhea.After treated with ultra-micro powder Qiweibaizhusan,traditional Chinese medicine dachengqitang group were cured quickly and the symptom of insufficiency of the spleen disappeared.The quantities of intestinal lactobacillus and fungus in antibiotic group were much higher than the normal group（P〈0.01）.[Conclusion] A dysbacteriotic mice modeling method of diarrhea was constructed.There were some kinds of probiotics contained in ultra-micro powder Qiweibaizhusan which could promote the growth of intestinal lactobacillus and fungus.

关 键 词：腹泻模型 肠道微生物 七味白术散 中医药微生态 

分 类 号：R285.5[医药卫生—中药学]