染料高效脱色光合细菌的分离与分析  

Separation and analysis of photosynthetic bacteria of high efficiency decolorization

作　　者：李彦芹[1] 昌艳萍[1] 李春青[1] 陈涵茜[1] 康现江[1] 

机构地区：[1]河北大学生命科学学院,河北保定071002

出　　处：《河北大学学报（自然科学版）》2012年第4期399-405,共7页Journal of Hebei University(Natural Science Edition)

基　　金：河北省自然科学基金资助项目(C2011201028)

摘　　要：为增强染料废水的生物处理,从印染厂的污泥中分离筛选出1株高效脱色光合细菌HL.对其在不同pH、碳源和氮源的条件下对5种染料的脱色效果进行了研究.结果表明,菌株HL菌落呈鲜红色,圆形、光滑、湿润、稍突起、边缘整齐,直径0.5～2.0mm;个体呈螺旋状,单根极生鞭毛,革兰氏染色为阴性,含有细菌叶绿素a,可进行光合作用.对活性艳红和直接胡兰的脱色效果显著,特别是对直接胡兰在24h内脱色率达到100%.菌株HL在中性环境下,分别以葡萄糖和氯化铵作为脱色培养基的碳源和氮源时,对直接胡兰的脱色率和降解率分别达到100%和98.67%.In order to enhance the biological treatment of dyestuff wastewater, a strain HL of photosynthetic bacteria with high efficiency on the dye decoloring ability were separated and analyzed. The effects of the strain HL on decolourization of five dyes under different conditions such as the choice of pH, carbon source and nitrogen source were studied. Results showed that the strain HL colony was bright red, circular, moist, smooth, slightly protuberant, edge tidy, diameter 0.5--2.0 mm, individual assume spiral-shaped, single extremely born flagellum, gram＇s staining for negative, contain bacteria chlorophyll-a for photosynthesis. And the decoloring ability were determined on the five types of dye , including on the active liquor and direct Hulan of decolorization optimal, especially for the treatment of ability within 24 h on direct Hulan was 100%. Strain HL in a neutral environment, respectively with glucose and ammonium chloride as decoloring medium carbon source and nitrogen source, the decolored rate and degradation rate of direct Hulan were 100% and 98.67% respectively.

关 键 词：光合细菌 印染废水 脱色率 降解率 

分 类 号：Q939.9[生物学—微生物学]