基因克隆策略在抗稻瘟病基因分离中的应用  

Applications of Gene Cloning Strategies in the Rice Blast Resistance Gene Isolation

作　　者：刘杨[1,2] 肖应辉[1,2] 史学涛[1,2] 刘雄伦[1,2] 戴良英[2,3] 王国梁[1,2] 

机构地区：[1]湖南农业大学农学院,长沙410128 [2]作物基因工程湖南省重点实验室,长沙410128 [3]湖南农业大学生物安全学院,长沙410128

出　　处：《中国农学通报》2012年第24期166-172,共7页Chinese Agricultural Science Bulletin

基　　金：国家自然科学基金"水稻广谱抗稻瘟病基因pi47和pi48的精细定位和克隆"(31171834);国家自然科学基金"籼稻品种Jefferson中广谱抗瘟基因的图位克隆与进化分析"(31171526)

摘　　要：稻瘟病是限制水稻生产的主要病害之一,不断分离克隆抗性基因并通过现代生物技术培育抗病水稻新品种是防治该病最经济有效的途径。介绍了当前4种主要的基因克隆即转座子标签法、图位克隆法、电子克隆法和电子图位克隆法的原理和操作流程,并概述了已克隆的19个稻瘟病抗性基因的克隆途径,分别举例介绍了抗稻瘟病基因图位克隆策略、电子图位克隆策略和转座子标签法克隆策略的主要流程。结合研究实践,提出稻瘟病基因的克隆要在不断通过对抗病基因结构和功能深入了解的基础上,灵活选择不同基因分离策略,发挥多种克隆策略的长处。Rice blast, caused by Magnaportbe oryzae, is one of the most destructive diseases that limits rice production worldwide. Use of blast resistance genes is considered as the most economical and environment-friendly method to control the rice blast disease. In this paper, we discussed the principles and procedures of four major gene cloning technologies, which included transposon tagging, map-based cloning, in silico cloning and in silico map-based cloning. The cloning approaches for the 19 resistance genes to M. oryzae were summarized. The widely-used three cloning strategies were discussed in details with examples. To efficiently clone a tagged resistance gene, we should first decide the cloning strategy according to the origin and chromosomal location of the gene and availability of mapping populations and also combine different strategies if needed.

关 键 词：水稻 稻瘟病 抗病基因 基因克隆 策略 

分 类 号：Q945.8[农业科学—植物病理学]