乳酸片球菌的分离鉴定与益生功能的初步研究  

Isolation,Identification and the Preliminary Study on the Healthy Function of Pediococcus acidilactici

作　　者：王建设[1] 赵海燕[2] 王凡[1] 霍征宇 马文胜 钱东明[1] 王安如[1] 

机构地区：[1]北京大北农科技集团股份有限公司,饲用微生物工程国家重点实验室,北京100193 [2]山西农业大学农学院,山西太谷030801 [3]内蒙古乌兰察布市动物疾病预防控制中心,内蒙古乌兰察布012000

出　　处：《中国农学通报》2012年第24期201-206,共6页Chinese Agricultural Science Bulletin

基　　金："十二五"国家科技攻关项目"饲用乳酸菌应用技术集成与推广示范"(NC2010CA0199);国家"973"项目"有益微生物对动物的生物学活性及功能研究"(2010CB35703);北京市海淀区重大科技研发资助项目"新型饲用乳酸菌产品的研制与产业化"(K20100130Z)

摘　　要：为了获取作为微生态制剂的候选菌株。本研究从鸭的空肠内容物中分离到22株乳酸菌,并对菌株ZW001进行研究。通过形态、生理生化、16SrDNA序列分析及系统发育树构建,鉴定为Pediococcus acidilactic(i乳酸片球菌)。而且从耐酸、耐胆盐、耐高温及抑菌方面,研究其益生功能。结果表明:乳酸片球菌ZW001具有较好的抗逆性和抑菌特性。总之,菌株ZW001可以作为益生菌的候选菌株应用于饲料工业。In order to obtain the candidate strains of probiotics, twenty-two lactic acid bacteria were isolated from the jejunum content of ducks, and the strain ZW001 was researched in this article. According to the results of morphological observation, physiological and biochemical test, 16S rDNA sequence homology analysis, the strain ZW001 was identified as Pediococcus acidilactici. Moreover, the healthy function of the strain, such as acid resistance, bile salt tolerance, high temperature tolerance and antibacterial activity, was researched. The results showed that strain ZWO01 had good the stress tolerance, and exhibited good antibacterial activity. In conclusion, these properties made the strain ZW001 promising for application in the feed industry.

关 键 词：益生菌 乳酸片球菌 耐酸性 耐胆盐 益生功能 

分 类 号：Q93[生物学—微生物学]