鹅源黄病毒的分离和初步鉴定  

Isolation and Preliminary Identification of a Novel Flavivirus Strain from Geese

作　　者：袁生[1] 李金平[1,2] 王敏儒[1] 白挨泉[1] 蒲文珺[1] 张济培[1] 彭巧丽[2] 王辉[2] 陈志伟[2,3] 张浩吉[1] 

机构地区：[1]佛山科学技术学院动物医学系,广东佛山528231 [2]香港大学李嘉诚医学院艾滋病研究所深圳研究室,深圳第三人民医院,广东深圳518112 [3]香港大学李嘉诚医学院艾滋病研究所,中国香港

出　　处：《中国畜牧兽医》2012年第9期47-50,共4页China Animal Husbandry & Veterinary Medicine

基　　金：广东省自然科学基金项目(10152800001000007);广东省农业攻关计划项目(2005B20201008)

摘　　要：本试验从广东地区临床表现为体温升高、采食量减少、拉稀粪、站立不稳、运动障碍的幼龄病鹅群,无菌采集病料,进行病原的分离传代。研究结果表明,该病毒对番鸭胚的半数致死量(ELD50)为10-2.68/0.2mL,在pH 7.2的条件下不能凝集鸭、鸡、鹅、鸽的红细胞,人工感染15日龄健康幼鹅能复制出与自然病例相同的临床症状和病变。用BYD病毒E基因片段的特异性引物,对试验获得毒株进行RT-PCR扩增和序列分析,证明获得的序列与GenBank中收录的发现于中国引起鸭产蛋下降综合征的新型黄病毒—BYD病毒(登录号为JF312912)和中国江苏发现的鹅黄病毒JS804株(登录号为JF895923)的同源性最高(有2个碱基的差异),均达到了99%以上,对试验获得的毒株暂定为鹅源BYD病毒广东株1(简称为BYD-GD1)。A virus strain isolated from young geese（gosling）with fever,feed uptake decline,diarrhea,standing instability and dyskinesia signs,in Guangdong area.The isolated virus ELD_50 to muscovry duck was 10-2.68/0.2 mL,and had no hemagglutination activity on chichen,duck,goose or pigeon sera at pH 7.2.A animal regression experiment was conducted,and a similar disease was reproduced in 15-day healthy geese.Using specific PCR primer pairs,which was used to amplified partial E gene of the BYD virus,a 401 bp nucleotide fragment was obtained by RT-PCR.The obtained sequence was compared to the GenBank database using BLAST and the closest sequences were BYD virus（GenBank accession JF312912）,which caused duck egg-drop syndrome in China,and a novel flavivirus strain JS804（GenBank accession JF895923）,which isolated from geese in Jiangsu China,with a similarity of 99%.These results suggested that the isolated virus from geese in Guangdong was the BYD virus of flavivirus genus and called a brief name for BYD-GD1.

关 键 词：黄病毒 鹅 分离 鉴定 

分 类 号：S852.65[农业科学—基础兽医学]