应用逆转录套式PCR检测猪流行性腹泻病毒  

Detection of Porcine Epidemic Diarrhea Virus by RT-nested PCR

作　　者：王金良[1] 谢金文[1] 唐娜[1] 祖立闯 李娇 汪洋 沈志强[1,2] 

机构地区：[1]山东省滨州畜牧兽医研究院,山东滨州256600 [2]山东绿都生物科技有限公司,山东滨州256600

出　　处：《中国畜牧兽医》2012年第9期86-88,共3页China Animal Husbandry & Veterinary Medicine

基　　金：山东省自然科学基金资助项目(ZR2010CQ012)

摘　　要：根据已发表的猪流行性腹泻病毒(PEDV)N基因核苷酸序列,设计合成2对引物,其中外引物扩增片段长度为297bp,内引物扩增片段长度为212bp,在优化RT-PCR反应条件的基础上,建立了检测PEDV的套式RT-PCR检测方法,该方法较普通RT-PCR更加灵敏、可靠,能有效降低假阳性和污染,可用于PEDV的快速诊断和流行病学调查。Based on the published nucleotide sequence of porcine epidemic diarrhea virus N gene,two pairs of primers were designed and synthesized,which outer primers amplified fragment length was 297 bp,and inner primers amplified fragment length was 212 bp.RT-nested PCR methods had been established through optimization of the RT-PCR reaction conditions,this method was more sensitive and reliable than ordinary RT-PCR,and could effectively reduce the false positive and pollution.It could be used for rapid diagnosis and epidemiological investigation of the PEDV.

关 键 词：猪流行性腹泻病毒 N基因 套式RT-PCR 检测 

分 类 号：S852.629.7[农业科学—基础兽医学]