彝族酒精依赖患者乙醇脱氢酶2基因多态性研究  

The gene polymorphism of ADH2 of alcohol dependence pa- tients of Yi nationality

作　　者：鲍建军[1] 武文煜[1] 张旭兰[1] 

机构地区：[1]云南省精神病医院,云南昆明650224

出　　处：《临床心身疾病杂志》2012年第5期392-393,共2页Journal of Clinical Psychosomatic Diseases

基　　金：云南省自然科学基金资助项目（编号2009CD080）

摘　　要：目的探讨昆明彝族地区酒精依赖患者乙醇脱氢酶2基因多态性，为揭示酒精依赖的发病机制和临床治疗提供依据。方法将98例彝族酒精依赖患者设为患者组，抽取同期同地区215名彝族健康志愿者设为对照组，采用多聚酶链式反应限制性长度片段多态性技术测定两组被试乙醇脱氢酶2基因多态性，对两组基因型分布及等位基因频率测定结果进行对比分析。结果患者组乙醇脱氢酶2＊1／乙醇脱氢酶2＊1基因型分布及乙醇脱氢酶2＊1等位基因频率均显著高于对照组（P〈0．01）；乙醇脱氢酶2＊2／乙醇脱氢酶2＊2基因型分布及乙醇脱氢酶2＊2等位基因频率显著低于对照组（P〈0．01）。结论酒精依赖患者乙醇脱氢酶2＊1等位基因频率明显升高，而乙醇脱氢酶2＊2等位基因频率明显下降；乙醇脱氢酶2＊2等位基因是酒精依赖发病的重要影响因素。Objective To explore the gene polymorphism of alcohol dehydrogen- ase 2 （ADH2） of alcohol dependence （AD） patients of Yi nationality in order toprovide basis for revealig the pathogenesis of AD and clinical treatment. Methods Ninety eight AD pa- tients of Yi nationality were assigned to patient group and 215 healthy volunteers of the same nationality selected contempo}aneously from the same district to control group, the gene polymorphisms of ADH2 measured using the polymerase chain reaction-restriction fragment length polymorphisms （PCR-RFLP）, and determination results of genotype distribution and allele frequency eontrastively analyzed between two groups. Results The freaquency of ADH2＊ 1/1 genotype distribution and ADH2 ＊ 1 allele was signifi- cantly higher （P〈0.01） and that of ADH2 ＊ 2/2 genotype distribution and ADH2＊ 2 allele lower（P〈0.01） in patient than in control group. Conclusion The frequency of ADH2 ＊ 1 allele heightens notably and that of ADH2 ＊ 2 allele lowers in AD patients; ADH2 ~ 2 allele is an important influencing factor in developing alcoholism.

关 键 词：酒精依赖 乙醇脱氢酶2 基因多态性 基因型分布频率 等位基因频率 发病机制 

分 类 号：R749.62[医药卫生—神经病学与精神病学]