野八角果实中的倍半萜和木脂素及其细胞毒活性(英文)  

Sesquiterpenes and lignans from the fruits of Illicium simonsii and their cytotoxicities

作　　者：尹鹏军[1] 汪俊松[1] 王朋然[1] 孔令义[1] 

机构地区：[1]中国药科大学天然药物化学教研室,南京210009

出　　处：《中国天然药物》2012年第5期383-387,共5页

基　　金：supported by the Key Project of National Natural Science Foundation of China (No. 30830116);Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)~~

摘　　要：目的:研究野八角果实的化学成分及生物活性。方法:应用硅胶、反相、凝胶色谱及制备 HPLC 对野八角果实的乙醇提取物进行分离纯化, 并且通过光谱方法鉴定化合物结构。进一步用 MTT 法对所分离化合物进行细胞毒活性测试。结果:7 个化合物被分离并鉴定为:veranisatin F (1), anisatin (2), (1S)-minwanenone (3), honokiol (4), macranthol (5), (+)-pinoresinol (6),matairesinol (7)。结论:化合物 1 是一个新的高氧化裂-prezizaane 型倍半萜, 化合物 6 和 7 首次从野八角中分离得到。5-氟脲嘧啶作为阳性对照, 化合物 2 和 3 对人大细胞肺癌细胞株和人肝癌细胞株的增长有较强的的抑制活性, 7 则比较微弱, 其他化合物显示无细胞毒活性。AIM： To study the constituents and bioactivity of Illicium simonsii. METHODS： Compounds were isolated by a com- bination of chromatographic materials, silica gel, ODS, and Sephadex LH-20, and finally purified by preparative HPLC. Their struc- tures were elucidated on the basis of spectroscopic methods, and they were evaluated for their cytotoxic activities by the MTF method. RESULTS： Seven compounds were obtained and were identified as veranisatin F （1）, anisatin （2）, （1S）-minwanenone （3）, honokiol （4）, macranthol （5）, （＋）-pinoresinol （6）, and matairesinol （7）. CONCLUSIONS： Compound 1 is a new seco-prezizaane-type sesquiterpene, while compounds 6 and 7 were isolated from Illiciurn simonsii for the first time. Compounds 2 and 3 showed strong inhibitory activities against the growth of NCI-H460 and SMMC-7721 cells comparable to 5-fluorouracil, and compound 7 to a lesser extent; the other isolates showed no cytotoxicity.

关 键 词：八角属 野八角 高氧化裂-prezizaane型倍半萜 木脂素 细胞毒 

分 类 号：R284.1[医药卫生—中药学]