反相高效液相色谱法测定榆黄酊剂中大黄素、大黄酚的含量  

Determination of Emodin and Chrysophanol in Yuhuang Tincture by RP-HPLC

作　　者：戴颖[1] 江宝红[2] 黄一平[1] 

机构地区：[1]江苏省中医药研究院,江苏南京210028 [2]南京中医药大学,江苏南京210046

出　　处：《亚太传统医药》2012年第9期25-27,共3页Asia-Pacific Traditional Medicine

基　　金：江苏省中医药局立项课题(L09068)

摘　　要：目的:建立榆黄酊剂中大黄素、大黄酚的含量测定方法。方法:采用反相高效液相色谱法(RP-HPLC)法,色谱柱为Alltima C18(4.6mm×250mm,5μm);流动相为甲醇-0.1%磷酸溶液(78:22);流速为1.0mL·min-1;柱温35℃,进样量10μL。结果:大黄素在0.059～0.588μg/mL内峰面积积分值与进样量自然对数有良好的线性关系线,r=0.999 9;平均加样回收率为98.1%,RSD为1.25%,大黄酚在0.210～2.10μg/mL内峰面积积分值与进样量有良好的线性关系线,r=0.999 9;平均加样回收率为97.7%,RSD为1.27%。结论:该方法操作简单,准确,重复性好,可用于榆黄酊剂的质量控制。Objective ： To determine astragaloside in Yu huang Tincture. Methods. The sanmple will determined on a Alltima C18 （4.6nm × 250ram, 5μm） by HPLC, the mobile phase consisted methanol-0.1% phosphoric acid solution（13：87）with flower rate at 1.0 mL·min^-1 and column temperature at 35℃, and injection voulm was 10μL. Results：The linear ranges of emodin was 0. 059- 0. 588μg/mL（r= 0. 999 9） ,with the average recovery were 98.1% （RSD1.25%）, The linear ranges of chrysophanol was 0. 210-2.10ffg/mL（r = 0. 999 9）,with the average recovery were 97.7% （RSD1.27%）. Conclusion： The method is simple, accurate, reproducible and it can be used for the quality control of Yu huang Tincture.

关 键 词：反高效液相 榆黄酊剂 大黄素 大黄酚 

分 类 号：R284.2[医药卫生—中药学]