半边旗中抗肿瘤成分6F的分离纯化  

Isolation and Purification of an Active Antitumor Compound 6F from Pteris semipinnata L.

作　　者：龚先玲[1] 梁念慈[1] 邓亦峰[1] 

机构地区：[1]广东医学院天然药物研究与开发重点实验室,广东湛江524023

出　　处：《亚太传统医药》2012年第9期29-30,共2页Asia-Pacific Traditional Medicine

基　　金：广东省中医药局科研课题(2009235)

摘　　要：目的:分离纯化半边旗中抗肿瘤活性成分6F(即7β,9-二羟基-15-氧-对映-贝壳杉烷-16-烯-19,6β-内酯)。方法:采用二氧化碳超临界萃取、结晶与重结晶、硅胶柱层析方法进行分离、纯化6F;采用HPLC-MS、HPLC和TLC检测6F的纯度。结果:经HPLC和TLC检测,可以得到6F的纯化合物。结论:采用结晶与重结晶、硅胶柱层析方法,可有效纯化半边旗中抗肿瘤成分6F。Objective： To obtain an active antitumor compound 6F （7β, 9- dihydroxy-15-oxo-ent-kaur-16-en-19,6β-olide） from Pteris semipinnata L.. Methods： Supercritical carbon dioxide extraction, crystallization, recrystallization and column chromatogra- phy were used to isolate and purify the compound 6F. The purity of 6F was detected by HPLC-MS, HPLC and TLC. Results： The purified compound 6F was confirmed by HPLC and TLC. Conclusion：The antitumor compound 6F is obtained by the isolation and purification using methods above.

关 键 词：半边旗 6F 6F纯化 

分 类 号：R284[医药卫生—中药学]