多囊卵巢综合征大鼠卵巢中分泌性白细胞蛋白酶抑制因子的表达  

Expression of secretory leukocyte protease inhibitor in rats with polycystic ovary syndrome

作　　者：周礼兰[1] 刘继晓[1] 马葆荣[1] 林婉君[1] 

机构地区：[1]天津市中心妇产科医院妇科,300100

出　　处：《国际内分泌代谢杂志》2012年第5期299-302,F0003,共5页International Journal of Endocrinology and Metabolism

摘　　要：目的探讨分泌性白细胞蛋白酶抑制因子（SLPI）在多囊卵巢综合征（PCOS）大鼠模型卵巢中的表达及生物学作用。方法16只雌性Sprague—Dawley（SD）大鼠随机分为实验组和正常对照组（各8只）。实验组以胰岛素联合人绒毛膜促性腺激素法建立SD大鼠的PCOS模型，正常对照组注射生理盐水。观察两组大鼠卵巢形态、动情周期等变化。测定血清黄体生成素、睾酮、胰岛素、雌激素。免疫组化法分析两组大鼠卵巢中SLPI的表达，RT—PCR及Western印迹技术检测并比较两组大鼠SLPImRNA及蛋白质的表达。结果实验组大鼠卵巢形态及激素变化符合PCOS的诊断，免疫组化结果显示实验组较正常对照组大鼠SLPI表达增加，多囊卵巢的卵巢基质、黄体细胞、卵泡的颗粒层及膜中皆有SLPI表达。实验组大鼠卵巢SLPI的基因（P〈0．05）及蛋白表达（P〈0．05）均高于正常对照组大鼠。结论多囊卵巢中SLPI的表达增加可能与PCOS大鼠卵巢功能修复有关。Objective To study the expression and biological effects of secretory leukocyte proteas inhibitor （SLPI） in polycystic ovary syndrome （PCOS） of rat model. Methods Sixteen female Sprague Dawley（SD） rats were divided into two groups： experimental group and normal control group（8 in eacl group）. PCOS were induced in experimental group by combination of insulin and human chorionic gonadotro phin, while rats in control group were injected with physiological saline. The ovary morphological changes an, estrous cycle were observed and compared. Serum luteinizing hormone, testosterone, insulin, estrogen level were measured. The expression of SLPI in the ovary of each group were detected by immunohistochemistry and SLPI mRNA and protein were measured and compared by RT-PCR and Western blot. Results Th, changes of ovary morphology and sex hormone levels conformed to clinical PCOS. Immunohistochemical anal ysis showed that SLPI expression was higher in the ovarian stroma, corpus luteum, and granulose layer of th~ follicle in polycystic ovary （PCO） compared with that in the normal ovary. The levels of SLPI mRNA an~ protein were higher in experimental group than those in the normal control group. Conclusion An increas, of SLPI expression in PCO may be related to the suppression of ovary disruption in rats with PCOS.

关 键 词：多囊卵巢综合征 分泌性白细胞蛋白酶抑制因子 组织修复 

分 类 号：R711.75[医药卫生—妇产科学]